4mys: Difference between revisions

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==1.4 Angstrom Crystal Structure of 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase with SHCHC and Pyruvate==
==1.4 Angstrom Crystal Structure of 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase with SHCHC and Pyruvate==
<StructureSection load='4mys' size='340' side='right' caption='[[4mys]], [[Resolution|resolution]] 1.42&Aring;' scene=''>
<StructureSection load='4mys' size='340' side='right'caption='[[4mys]], [[Resolution|resolution]] 1.42&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[4mys]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MYS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4MYS FirstGlance]. <br>
<table><tr><td colspan='2'>[[4mys]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MYS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4MYS FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=164:2-(3-CARBOXYPROPIONYL)-6-HYDROXY-CYCLOHEXA-2,4-DIENE+CARBOXYLIC+ACID'>164</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PYR:PYRUVIC+ACID'>PYR</scene></td></tr>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=164:2-(3-CARBOXYPROPIONYL)-6-HYDROXY-CYCLOHEXA-2,4-DIENE+CARBOXYLIC+ACID'>164</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PYR:PYRUVIC+ACID'>PYR</scene></td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4mxd|4mxd]], [[4myd|4myd]]</td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[4mxd|4mxd]], [[4myd|4myd]]</div></td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">b2263, JW2258, menH, yfbB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate_synthase 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.99.20 4.2.99.20] </span></td></tr>
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate_synthase 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.99.20 4.2.99.20] </span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4mys FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mys OCA], [https://pdbe.org/4mys PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4mys RCSB], [https://www.ebi.ac.uk/pdbsum/4mys PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4mys ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4mys FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mys OCA], [http://pdbe.org/4mys PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4mys RCSB], [http://www.ebi.ac.uk/pdbsum/4mys PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4mys ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[http://www.uniprot.org/uniprot/MENH_ECOLI MENH_ECOLI]] Catalyzes a proton abstraction reaction that results in 2,5-elimination of pyruvate from 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) and the formation of 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate (SHCHC). Is also able to catalyze the hydrolysis of the thioester bond in palmitoyl-CoA in vitro.<ref>PMID:15808744</ref> <ref>PMID:18284213</ref>   
[[https://www.uniprot.org/uniprot/MENH_ECOLI MENH_ECOLI]] Catalyzes a proton abstraction reaction that results in 2,5-elimination of pyruvate from 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) and the formation of 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate (SHCHC). Is also able to catalyze the hydrolysis of the thioester bond in palmitoyl-CoA in vitro.<ref>PMID:15808744</ref> <ref>PMID:18284213</ref>   
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== Publication Abstract from PubMed ==
== Publication Abstract from PubMed ==
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</StructureSection>
</StructureSection>
[[Category: 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase]]
[[Category: 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase]]
[[Category: Ecoli]]
[[Category: Large Structures]]
[[Category: Feng, Y]]
[[Category: Feng, Y]]
[[Category: Guo, Z]]
[[Category: Guo, Z]]

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