1hx8: Difference between revisions

Revision as of 19:19, 2 May 2008

CRYSTAL STRUCTURE OF N-TERMINAL DOMAIN OF DROSOPHILA AP180

OverviewOverview

Clathrin-mediated endocytosis plays a major role in retrieving synaptic vesicles from the plasma membrane following exocytosis. This endocytic process requires AP180 (or a homolog), which promotes the assembly and restricts the size of clathrin-coated vesicles. The highly conserved 33 kDa amino-terminal domain of AP180 plays a critical role in binding to phosphoinositides and in regulating the clathrin assembly activity of AP180. The crystal structure of the amino-terminal domain reported herein reveals a novel fold consisting of a large double layer of sheets of ten alpha helices and a unique site for binding phosphoinositides. The finding that the clathrin-box motif is mostly buried and lies in a helix indicates a different site and mechanism for binding of the domain to clathrins than previously assumed.

About this StructureAbout this Structure

1HX8 is a Single protein structure of sequence from Drosophila melanogaster. Full crystallographic information is available from OCA.

ReferenceReference

A novel all helix fold of the AP180 amino-terminal domain for phosphoinositide binding and clathrin assembly in synaptic vesicle endocytosis., Mao Y, Chen J, Maynard JA, Zhang B, Quiocho FA, Cell. 2001 Feb 9;104(3):433-40. PMID:11239400 Page seeded by OCA on Fri May 2 19:19:13 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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 [[Image:1hx8.jpg|left|200px]]
- {{Structure
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-|GENE= LAP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7227 Drosophila melanogaster])
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-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1hx8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hx8 OCA], [http://www.ebi.ac.uk/pdbsum/1hx8 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1hx8 RCSB]</span>
-}}
 '''CRYSTAL STRUCTURE OF N-TERMINAL DOMAIN OF DROSOPHILA AP180'''
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 [[Category: Quiocho, F A.]]
 [[Category: Zhang, B.]]
-[[Category: all alpha]]
+[[Category: All alpha]]
-[[Category: alpha helices repeat]]
+[[Category: Alpha helices repeat]]
-[[Category: coiled-coil]]
+[[Category: Coiled-coil]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 19:19:13 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:11:36 2008''