3x05: Difference between revisions

From Proteopedia
Jump to navigation Jump to search
← Older editNewer edit →
No edit summary
No edit summary
Line 1: Line 1:


==Crystal structure of PIP4KIIBETA T201M complex with AMP==
==Crystal structure of PIP4KIIBETA T201M complex with AMP==
<StructureSection load='3x05' size='340' side='right' caption='[[3x05]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
<StructureSection load='3x05' size='340' side='right'caption='[[3x05]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[3x05]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3X05 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3X05 FirstGlance]. <br>
<table><tr><td colspan='2'>[[3x05]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3X05 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3X05 FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene></td></tr>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene></td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3wzz|3wzz]], [[3x01|3x01]], [[3x02|3x02]], [[3x03|3x03]], [[3x04|3x04]], [[3x06|3x06]], [[3x07|3x07]], [[3x08|3x08]], [[3x09|3x09]], [[3x0a|3x0a]], [[3x0b|3x0b]], [[3x0c|3x0c]]</td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3wzz|3wzz]], [[3x01|3x01]], [[3x02|3x02]], [[3x03|3x03]], [[3x04|3x04]], [[3x06|3x06]], [[3x07|3x07]], [[3x08|3x08]], [[3x09|3x09]], [[3x0a|3x0a]], [[3x0b|3x0b]], [[3x0c|3x0c]]</div></td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PIP4K2B ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PIP4K2B ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/1-phosphatidylinositol-5-phosphate_4-kinase 1-phosphatidylinositol-5-phosphate 4-kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.149 2.7.1.149] </span></td></tr>
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/1-phosphatidylinositol-5-phosphate_4-kinase 1-phosphatidylinositol-5-phosphate 4-kinase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.149 2.7.1.149] </span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3x05 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3x05 OCA], [http://pdbe.org/3x05 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3x05 RCSB], [http://www.ebi.ac.uk/pdbsum/3x05 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3x05 ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3x05 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3x05 OCA], [https://pdbe.org/3x05 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3x05 RCSB], [https://www.ebi.ac.uk/pdbsum/3x05 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3x05 ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[http://www.uniprot.org/uniprot/PI42B_HUMAN PI42B_HUMAN]] Participates in the biosynthesis of phosphatidylinositol 4,5-bisphosphate.<ref>PMID:9038203</ref>   
[[https://www.uniprot.org/uniprot/PI42B_HUMAN PI42B_HUMAN]] Participates in the biosynthesis of phosphatidylinositol 4,5-bisphosphate.<ref>PMID:9038203</ref>   
<div style="background-color:#fffaf0;">
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
== Publication Abstract from PubMed ==
Line 27: Line 27:
[[Category: 1-phosphatidylinositol-5-phosphate 4-kinase]]
[[Category: 1-phosphatidylinositol-5-phosphate 4-kinase]]
[[Category: Human]]
[[Category: Human]]
[[Category: Large Structures]]
[[Category: Asara, J A]]
[[Category: Asara, J A]]
[[Category: Cantley, L C]]
[[Category: Cantley, L C]]

Revision as of 08:37, 10 August 2022

Crystal structure of PIP4KIIBETA T201M complex with AMPCrystal structure of PIP4KIIBETA T201M complex with AMP

Structural highlights

3x05 is a 2 chain structure with sequence from Human. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Gene:PIP4K2B (HUMAN)
Activity:1-phosphatidylinositol-5-phosphate 4-kinase, with EC number 2.7.1.149
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[PI42B_HUMAN] Participates in the biosynthesis of phosphatidylinositol 4,5-bisphosphate.[1]

Publication Abstract from PubMed

While cellular GTP concentration dramatically changes in response to an organism's cellular status, whether it serves as a metabolic cue for biological signaling remains elusive due to the lack of molecular identification of GTP sensors. Here we report that PI5P4Kbeta, a phosphoinositide kinase that regulates PI(5)P levels, detects GTP concentration and converts them into lipid second messenger signaling. Biochemical analyses show that PI5P4Kbeta preferentially utilizes GTP, rather than ATP, for PI(5)P phosphorylation, and its activity reflects changes in direct proportion to the physiological GTP concentration. Structural and biological analyses reveal that the GTP-sensing activity of PI5P4Kbeta is critical for metabolic adaptation and tumorigenesis. These results demonstrate that PI5P4Kbeta is the missing GTP sensor and that GTP concentration functions as a metabolic cue via PI5P4Kbeta. The critical role of the GTP-sensing activity of PI5P4Kbeta in cancer signifies this lipid kinase as a cancer therapeutic target.

The Lipid Kinase PI5P4Kbeta Is an Intracellular GTP Sensor for Metabolism and Tumorigenesis.,Sumita K, Lo YH, Takeuchi K, Senda M, Kofuji S, Ikeda Y, Terakawa J, Sasaki M, Yoshino H, Majd N, Zheng Y, Kahoud ER, Yokota T, Emerling BM, Asara JM, Ishida T, Locasale JW, Daikoku T, Anastasiou D, Senda T, Sasaki AT Mol Cell. 2016 Jan 21;61(2):187-98. doi: 10.1016/j.molcel.2015.12.011. Epub 2016 , Jan 7. PMID:26774281[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

  1. ↑ Castellino AM, Parker GJ, Boronenkov IV, Anderson RA, Chao MV. A novel interaction between the juxtamembrane region of the p55 tumor necrosis factor receptor and phosphatidylinositol-4-phosphate 5-kinase. J Biol Chem. 1997 Feb 28;272(9):5861-70. PMID:9038203
  2. ↑ Sumita K, Lo YH, Takeuchi K, Senda M, Kofuji S, Ikeda Y, Terakawa J, Sasaki M, Yoshino H, Majd N, Zheng Y, Kahoud ER, Yokota T, Emerling BM, Asara JM, Ishida T, Locasale JW, Daikoku T, Anastasiou D, Senda T, Sasaki AT. The Lipid Kinase PI5P4Kbeta Is an Intracellular GTP Sensor for Metabolism and Tumorigenesis. Mol Cell. 2016 Jan 21;61(2):187-98. doi: 10.1016/j.molcel.2015.12.011. Epub 2016 , Jan 7. PMID:26774281 doi:http://dx.doi.org/10.1016/j.molcel.2015.12.011

3x05, resolution 2.50Å

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA
Retrieved from "https://proteopedia.openfox.io/wiki/index.php?title=3x05&oldid=3602712"