7phi: Difference between revisions
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==Human voltage-gated potassium channel Kv3.1 (with Zn)== | ==Human voltage-gated potassium channel Kv3.1 (with Zn)== | ||
<StructureSection load='7phi' size='340' side='right'caption='[[7phi]]' scene=''> | <StructureSection load='7phi' size='340' side='right'caption='[[7phi]], [[Resolution|resolution]] 3.10Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7PHI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7PHI FirstGlance]. <br> | <table><tr><td colspan='2'>[[7phi]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7PHI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7PHI FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7phi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7phi OCA], [https://pdbe.org/7phi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7phi RCSB], [https://www.ebi.ac.uk/pdbsum/7phi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7phi ProSAT]</span></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=PCF:1,2-DIACYL-SN-GLYCERO-3-PHOSHOCHOLINE'>PCF</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7phi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7phi OCA], [https://pdbe.org/7phi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7phi RCSB], [https://www.ebi.ac.uk/pdbsum/7phi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7phi ProSAT]</span></td></tr> | |||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Kv3 channels have distinctive gating kinetics tailored for rapid repolarization in fast-spiking neurons. Malfunction of this process due to genetic variants in the KCNC1 gene causes severe epileptic disorders, yet the structural determinants for the unusual gating properties remain elusive. Here, we present cryo-electron microscopy structures of the human Kv3.1a channel, revealing a unique arrangement of the cytoplasmic tetramerization domain T1 which facilitates interactions with C-terminal axonal targeting motif and key components of the gating machinery. Additional interactions between S1/S2 linker and turret domain strengthen the interface between voltage sensor and pore domain. Supported by molecular dynamics simulations, electrophysiological and mutational analyses, we identify several residues in the S4/S5 linker which influence the gating kinetics and an electrostatic interaction between acidic residues in alpha6 of T1 and R449 in the pore-flanking S6T helices. These findings provide insights into gating control and disease mechanisms and may guide strategies for the design of pharmaceutical drugs targeting Kv3 channels. | |||
Cryo-EM structure of the human Kv3.1 channel reveals gating control by the cytoplasmic T1 domain.,Chi G, Liang Q, Sridhar A, Cowgill JB, Sader K, Radjainia M, Qian P, Castro-Hartmann P, Venkaya S, Singh NK, McKinley G, Fernandez-Cid A, Mukhopadhyay SMM, Burgess-Brown NA, Delemotte L, Covarrubias M, Durr KL Nat Commun. 2022 Jul 15;13(1):4087. doi: 10.1038/s41467-022-29594-w. PMID:35840580<ref>PMID:35840580</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 7phi" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Burgess-Brown | [[Category: Burgess-Brown, N A]] | ||
[[Category: Castro-Hartmann P]] | [[Category: Castro-Hartmann, P]] | ||
[[Category: Chi G]] | [[Category: Chi, G]] | ||
[[Category: Duerr | [[Category: Duerr, K L]] | ||
[[Category: Fernandez-Cid A]] | [[Category: Fernandez-Cid, A]] | ||
[[Category: MacLean | [[Category: MacLean, E M]] | ||
[[Category: Marsden B]] | [[Category: Marsden, B]] | ||
[[Category: McKinley G]] | [[Category: McKinley, G]] | ||
[[Category: Mukhopadhyay | [[Category: Mukhopadhyay, S M.M]] | ||
[[Category: Pike | [[Category: Pike, A C.W]] | ||
[[Category: Qian P]] | [[Category: Qian, P]] | ||
[[Category: Sader K]] | [[Category: Sader, K]] | ||
[[Category: Singh | [[Category: Singh, N K]] | ||
[[Category: Venkaya S]] | [[Category: Venkaya, S]] | ||
[[Category: Channel]] | |||
[[Category: Membrane protein]] | |||
[[Category: Potassium channel]] | |||
[[Category: Tetramer]] | |||
[[Category: Transport protein]] | |||
[[Category: Voltage-gated]] | |||
Revision as of 08:09, 10 August 2022
Human voltage-gated potassium channel Kv3.1 (with Zn)Human voltage-gated potassium channel Kv3.1 (with Zn)
Structural highlights
Publication Abstract from PubMedKv3 channels have distinctive gating kinetics tailored for rapid repolarization in fast-spiking neurons. Malfunction of this process due to genetic variants in the KCNC1 gene causes severe epileptic disorders, yet the structural determinants for the unusual gating properties remain elusive. Here, we present cryo-electron microscopy structures of the human Kv3.1a channel, revealing a unique arrangement of the cytoplasmic tetramerization domain T1 which facilitates interactions with C-terminal axonal targeting motif and key components of the gating machinery. Additional interactions between S1/S2 linker and turret domain strengthen the interface between voltage sensor and pore domain. Supported by molecular dynamics simulations, electrophysiological and mutational analyses, we identify several residues in the S4/S5 linker which influence the gating kinetics and an electrostatic interaction between acidic residues in alpha6 of T1 and R449 in the pore-flanking S6T helices. These findings provide insights into gating control and disease mechanisms and may guide strategies for the design of pharmaceutical drugs targeting Kv3 channels. Cryo-EM structure of the human Kv3.1 channel reveals gating control by the cytoplasmic T1 domain.,Chi G, Liang Q, Sridhar A, Cowgill JB, Sader K, Radjainia M, Qian P, Castro-Hartmann P, Venkaya S, Singh NK, McKinley G, Fernandez-Cid A, Mukhopadhyay SMM, Burgess-Brown NA, Delemotte L, Covarrubias M, Durr KL Nat Commun. 2022 Jul 15;13(1):4087. doi: 10.1038/s41467-022-29594-w. PMID:35840580[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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