1hw1: Difference between revisions

Revision as of 19:17, 2 May 2008

THE FADR-DNA COMPLEX: TRANSCRIPTIONAL CONTROL OF FATTY ACID METABOLISM IN ESCHERICHIA COLI

OverviewOverview

In Escherichia coli, the expression of fatty acid metabolic genes is controlled by the transcription factor, FadR. The affinity of FadR for DNA is controlled by long chain acyl-CoA molecules, which bind to the protein and modulate gene expression. The crystal structure of FadR reveals a two domain dimeric molecule where the N-terminal domains bind DNA, and the C-terminal domains bind acyl-CoA. The DNA binding domain has a winged-helix motif, and the C-terminal domain resembles the sensor domain of the Tet repressor. The FadR.DNA complex reveals how the protein interacts with DNA and specifically recognizes a palindromic sequence. Structural and functional similarities to the Tet repressor and the BmrR transcription factors suggest how the binding of the acyl-CoA effector molecule to the C-terminal domain may affect the DNA binding affinity of the N-terminal domain. We suggest that the binding of acyl-CoA disrupts a buried network of charged and polar residues in the C-terminal domain, and the resulting conformational change is transmitted to the N-terminal domain via a domain-spanning alpha-helix.

About this StructureAbout this Structure

1HW1 is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

ReferenceReference

The FadR.DNA complex. Transcriptional control of fatty acid metabolism in Escherichia coli., Xu Y, Heath RJ, Li Z, Rock CO, White SW, J Biol Chem. 2001 May 18;276(20):17373-9. Epub 2001 Feb 13. PMID:11279025 Page seeded by OCA on Fri May 2 19:17:15 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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 [[Image:1hw1.jpg|left|200px]]
- {{Structure
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+The line below this paragraph, containing "STRUCTURE_1hw1", creates the "Structure Box" on the page.
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-|GENE= FADR ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])
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-|RELATEDENTRY=[[1hw2|1HW2]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1hw1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hw1 OCA], [http://www.ebi.ac.uk/pdbsum/1hw1 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1hw1 RCSB]</span>
-}}
 '''THE FADR-DNA COMPLEX: TRANSCRIPTIONAL CONTROL OF FATTY ACID METABOLISM IN ESCHERICHIA COLI'''
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 [[Category: White, S W.]]
 [[Category: Xu, Y.]]
-[[Category: helix bundle]]
+[[Category: Helix bundle]]
-[[Category: helix-turn-helix]]
+[[Category: Helix-turn-helix]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 19:17:15 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:11:14 2008''