3wq7: Difference between revisions
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==New crystal form of the hyperthermophilic family 12 endo-cellulase from Pyrococcus furiosus== | ==New crystal form of the hyperthermophilic family 12 endo-cellulase from Pyrococcus furiosus== | ||
<StructureSection load='3wq7' size='340' side='right' caption='[[3wq7]], [[Resolution|resolution]] 1.68Å' scene=''> | <StructureSection load='3wq7' size='340' side='right'caption='[[3wq7]], [[Resolution|resolution]] 1.68Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[3wq7]] is a 2 chain structure with sequence from [ | <table><tr><td colspan='2'>[[3wq7]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_43587 Atcc 43587]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WQ7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3WQ7 FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> | ||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3vgi|3vgi]], [[3wq0|3wq0]], [[3wq1|3wq1]]</td></tr> | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3vgi|3vgi]], [[3wq0|3wq0]], [[3wq1|3wq1]]</div></td></tr> | ||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">eglA ([ | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">eglA ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2261 ATCC 43587])</td></tr> | ||
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[ | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Cellulase Cellulase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.4 3.2.1.4] </span></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3wq7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wq7 OCA], [https://pdbe.org/3wq7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3wq7 RCSB], [https://www.ebi.ac.uk/pdbsum/3wq7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3wq7 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
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[[Category: Atcc 43587]] | [[Category: Atcc 43587]] | ||
[[Category: Cellulase]] | [[Category: Cellulase]] | ||
[[Category: Large Structures]] | |||
[[Category: Ishikawa, K]] | [[Category: Ishikawa, K]] | ||
[[Category: Kataoka, M]] | [[Category: Kataoka, M]] | ||
Revision as of 08:46, 3 August 2022
New crystal form of the hyperthermophilic family 12 endo-cellulase from Pyrococcus furiosusNew crystal form of the hyperthermophilic family 12 endo-cellulase from Pyrococcus furiosus
Structural highlights
Publication Abstract from PubMedThe hyperthermophilic glycoside hydrolase family endocellulase 12 from the archaeon Pyrococcus furiosus (EGPf; Gene ID PF0854; EC 3.2.1.4) catalyzes the hydrolytic cleavage of the beta-1,4-glucosidic linkage in beta-glucan in lignocellulose biomass. A crystal of EGPf was previously prepared at pH 9.0 and its structure was determined at an atomic resolution of 1.07 A. This article reports the crystallization of EGPf at the more physiologically relevant pH of 5.5. Structure determination showed that this new crystal form has the symmetry of space group C2. Two molecules of the enzyme are observed in the asymmetric unit. Crystal packing is weak at pH 5.5 owing to two flexible interfaces between symmetry-related molecules. Comparison of the EGPf structures obtained at pH 9.0 and pH 5.5 reveals a significant conformational difference at the active centre and in the surface loops. The interfaces in the vicinity of the flexible surface loops impact the quality of the EGPf crystal. A new crystal form of a hyperthermophilic endocellulase.,Kataoka M, Ishikawa K Acta Crystallogr F Struct Biol Commun. 2014 Jul;70(Pt 7):878-83. doi:, 10.1107/S2053230X14010930. Epub 2014 Jun 18. PMID:25005081[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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