3vzi: Difference between revisions
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==Crystal Structure of CRISPR-associated Protein== | ==Crystal Structure of CRISPR-associated Protein== | ||
<StructureSection load='3vzi' size='340' side='right' caption='[[3vzi]], [[Resolution|resolution]] 2.66Å' scene=''> | <StructureSection load='3vzi' size='340' side='right'caption='[[3vzi]], [[Resolution|resolution]] 2.66Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[3vzi]] is a 2 chain structure with sequence from [ | <table><tr><td colspan='2'>[[3vzi]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Xanop Xanop]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VZI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3VZI FirstGlance]. <br> | ||
</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">cas5d ([ | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">cas5d ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=360094 XANOP])</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3vzi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vzi OCA], [https://pdbe.org/3vzi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3vzi RCSB], [https://www.ebi.ac.uk/pdbsum/3vzi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3vzi ProSAT]</span></td></tr> | ||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
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</div> | </div> | ||
<div class="pdbe-citations 3vzi" style="background-color:#fffaf0;"></div> | <div class="pdbe-citations 3vzi" style="background-color:#fffaf0;"></div> | ||
==See Also== | |||
*[[CRISPR subtype I-C|CRISPR subtype I-C]] | |||
== References == | == References == | ||
<references/> | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | |||
[[Category: Xanop]] | [[Category: Xanop]] | ||
[[Category: Bae, E]] | [[Category: Bae, E]] | ||
Revision as of 22:11, 27 July 2022
Crystal Structure of CRISPR-associated ProteinCrystal Structure of CRISPR-associated Protein
Structural highlights
Publication Abstract from PubMedClustered regularly interspaced short palindromic repeats (CRISPRs) and CRISPR-associated (Cas) proteins form an RNA-mediated microbial immune system against invading foreign genetic elements. Cas5 proteins constitute one of the most prevalent Cas protein families in CRISPR-Cas systems and are predicted to have RNA recognition motif (RRM) domains. Cas5d is a subtype I-C-specific Cas5 protein that can be divided into two distinct subgroups, one of which has extra C-terminal residues while the other contains a longer insertion in the middle of its N-terminal RRM domain. Here, we report crystal structures of Cas5d from Streptococcus pyogenes and Xanthomonas oryzae, which respectively represent the two Cas5d subgroups. Despite a common domain architecture consisting of an N-terminal RRM domain and a C-terminal beta-sheet domain, the structural differences between the two Cas5d proteins are highlighted by the presence of a unique extended helical region protruding from the N-terminal RRM domain of X. oryzae Cas5d. We also demonstrate that Cas5d proteins possess not only specific endoribonuclease activity for CRISPR RNAs but also nonspecific double-stranded DNA binding affinity. These findings suggest that Cas5d may play multiple roles in CRISPR-mediated immunity. Furthermore, the specific RNA processing was also observed between S. pyogenes Cas5d protein and X. oryzae CRISPR RNA and vice versa. This cross-species activity of Cas5d provides a special opportunity for elucidating conserved features of the CRISPR RNA processing event. Conservation and Variability in the Structure and Function of the Cas5d Endoribonuclease in the CRISPR-Mediated Microbial Immune System.,Koo Y, Ka D, Kim EJ, Suh N, Bae E J Mol Biol. 2013 Oct 23;425(20):3799-810. doi: 10.1016/j.jmb.2013.02.032. Epub, 2013 Mar 7. PMID:23500492[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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