3vz9: Difference between revisions

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==Crystal structure of the chicken Spc24-Spc25 globular domain==
==Crystal structure of the chicken Spc24-Spc25 globular domain==
<StructureSection load='3vz9' size='340' side='right' caption='[[3vz9]], [[Resolution|resolution]] 1.03&Aring;' scene=''>
<StructureSection load='3vz9' size='340' side='right'caption='[[3vz9]], [[Resolution|resolution]] 1.03&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[3vz9]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Chick Chick]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VZ9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3VZ9 FirstGlance]. <br>
<table><tr><td colspan='2'>[[3vz9]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Chick Chick]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VZ9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3VZ9 FirstGlance]. <br>
</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3vza|3vza]]</td></tr>
</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3vza|3vza]]</div></td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SPC25 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9031 CHICK])</td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SPC25 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9031 CHICK])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3vz9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vz9 OCA], [http://pdbe.org/3vz9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3vz9 RCSB], [http://www.ebi.ac.uk/pdbsum/3vz9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3vz9 ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3vz9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vz9 OCA], [https://pdbe.org/3vz9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3vz9 RCSB], [https://www.ebi.ac.uk/pdbsum/3vz9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3vz9 ProSAT]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
<div style="background-color:#fffaf0;">
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</StructureSection>
</StructureSection>
[[Category: Chick]]
[[Category: Chick]]
[[Category: Large Structures]]
[[Category: Fukagawa, T]]
[[Category: Fukagawa, T]]
[[Category: Nishino, T]]
[[Category: Nishino, T]]

Revision as of 22:11, 27 July 2022

Crystal structure of the chicken Spc24-Spc25 globular domainCrystal structure of the chicken Spc24-Spc25 globular domain

Structural highlights

3vz9 is a 2 chain structure with sequence from Chick. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Gene:SPC25 (CHICK)
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Publication Abstract from PubMed

The kinetochore forms a dynamic interface with microtubules from the mitotic spindle during mitosis. The Ndc80 complex acts as the key microtubule-binding complex at kinetochores. However, it is unclear how the Ndc80 complex associates with the inner kinetochore proteins that assemble upon centromeric chromatin. Here, based on a high-resolution structural analysis, we demonstrate that the N-terminal region of vertebrate CENP-T interacts with the 'RWD' domain in the Spc24/25 portion of the Ndc80 complex. Phosphorylation of CENP-T strengthens a cryptic hydrophobic interaction between CENP-T and Spc25 resulting in a phospho-regulated interaction that occurs without direct recognition of the phosphorylated residue. The Ndc80 complex interacts with both CENP-T and the Mis12 complex, but we find that these interactions are mutually exclusive, supporting a model in which two distinct pathways target the Ndc80 complex to kinetochores. Our results provide a model for how the multiple protein complexes at kinetochores associate in a phospho-regulated manner.

CENP-T provides a structural platform for outer kinetochore assembly.,Nishino T, Rago F, Hori T, Tomii K, Cheeseman IM, Fukagawa T EMBO J. 2013 Feb 6;32(3):424-36. doi: 10.1038/emboj.2012.348. Epub 2013 Jan 18. PMID:23334297[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

  1. ↑ Nishino T, Rago F, Hori T, Tomii K, Cheeseman IM, Fukagawa T. CENP-T provides a structural platform for outer kinetochore assembly. EMBO J. 2013 Feb 6;32(3):424-36. doi: 10.1038/emboj.2012.348. Epub 2013 Jan 18. PMID:23334297 doi:http://dx.doi.org/10.1038/emboj.2012.348

3vz9, resolution 1.03Å

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