3vm5: Difference between revisions
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==Recombinant medaka fish alpha-amylase expressed in yeast Pichia pastoris== | ==Recombinant medaka fish alpha-amylase expressed in yeast Pichia pastoris== | ||
<StructureSection load='3vm5' size='340' side='right' caption='[[3vm5]], [[Resolution|resolution]] 2.85Å' scene=''> | <StructureSection load='3vm5' size='340' side='right'caption='[[3vm5]], [[Resolution|resolution]] 2.85Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[3vm5]] is a 1 chain structure with sequence from [ | <table><tr><td colspan='2'>[[3vm5]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Japanese_killifish Japanese killifish]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VM5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3VM5 FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3vm5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vm5 OCA], [https://pdbe.org/3vm5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3vm5 RCSB], [https://www.ebi.ac.uk/pdbsum/3vm5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3vm5 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
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==See Also== | ==See Also== | ||
*[[Amylase|Amylase]] | *[[Amylase 3D structures|Amylase 3D structures]] | ||
== References == | == References == | ||
<references/> | <references/> | ||
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</StructureSection> | </StructureSection> | ||
[[Category: Japanese killifish]] | [[Category: Japanese killifish]] | ||
[[Category: Large Structures]] | |||
[[Category: Mikami, B]] | [[Category: Mikami, B]] | ||
[[Category: Mizutani, K]] | [[Category: Mizutani, K]] | ||
Revision as of 21:52, 27 July 2022
Recombinant medaka fish alpha-amylase expressed in yeast Pichia pastorisRecombinant medaka fish alpha-amylase expressed in yeast Pichia pastoris
Structural highlights
Publication Abstract from PubMedThe medaka fish alpha-amylase was expressed and purified. The expression systems were constructed using methylotrophic yeast Pichia pastoris, and the recombinant proteins were secreted into the culture medium. Purified recombinant alpha-amylase exhibited starch hydrolysis activity. The optimal pH, denaturation temperature, and K(M) and V(max) values were determined; chloride ions were essential for enzyme activity. The purified protein was also crystallized and examined by X-ray crystallography. The structure has the (alpha/beta)(8) barrel fold, as do other known alpha-amylases, and the overall structure is very similar to the structure of vertebrate (human and pig) alpha-amylases. A novel expression plasmid was developed. Using this plasmid, high-throughput construction of an expression system by homologous recombination in P. pastoris cells, previously reported for membrane proteins, was successfully applied to the secretory protein. Structural and functional characterization of recombinant medaka fish alpha-amylase expressed in yeast Pichia pastoris.,Mizutani K, Toyoda M, Otake Y, Yoshioka S, Takahashi N, Mikami B Biochim Biophys Acta. 2012 May 18. PMID:22613096[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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