3vdy: Difference between revisions
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==B. subtilis SsbB/ssDNA== | ==B. subtilis SsbB/ssDNA== | ||
<StructureSection load='3vdy' size='340' side='right' caption='[[3vdy]], [[Resolution|resolution]] 2.80Å' scene=''> | <StructureSection load='3vdy' size='340' side='right'caption='[[3vdy]], [[Resolution|resolution]] 2.80Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[3vdy]] is a 7 chain structure with sequence from [ | <table><tr><td colspan='2'>[[3vdy]] is a 7 chain structure with sequence from [https://en.wikipedia.org/wiki/"vibrio_subtilis"_ehrenberg_1835 "vibrio subtilis" ehrenberg 1835]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VDY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3VDY FirstGlance]. <br> | ||
</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ssbB, ywpH, BSU36310 ([ | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ssbB, ywpH, BSU36310 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 "Vibrio subtilis" Ehrenberg 1835])</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3vdy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vdy OCA], [https://pdbe.org/3vdy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3vdy RCSB], [https://www.ebi.ac.uk/pdbsum/3vdy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3vdy ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
[[ | [[https://www.uniprot.org/uniprot/SSBB_BACSU SSBB_BACSU]] Not essential for replication of the chromosome but is required for optimal competence. Probably binds ssDNA and allows DNA recombination.<ref>PMID:14762004</ref> | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
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==See Also== | ==See Also== | ||
*[[Single-stranded DNA-binding protein|Single-stranded DNA-binding protein]] | *[[Single-stranded DNA-binding protein 3D structures|Single-stranded DNA-binding protein 3D structures]] | ||
== References == | == References == | ||
<references/> | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: | [[Category: Vibrio subtilis ehrenberg 1835]] | ||
[[Category: Large Structures]] | |||
[[Category: Alonso, J C]] | [[Category: Alonso, J C]] | ||
[[Category: Carrasco, B]] | [[Category: Carrasco, B]] | ||
Revision as of 21:40, 27 July 2022
B. subtilis SsbB/ssDNAB. subtilis SsbB/ssDNA
Structural highlights
Function[SSBB_BACSU] Not essential for replication of the chromosome but is required for optimal competence. Probably binds ssDNA and allows DNA recombination.[1] Publication Abstract from PubMedWe have investigated the structural, biochemical and cellular roles of the two single-stranded (ss) DNA-binding proteins from Bacillus subtilis, SsbA and SsbB. During transformation, SsbB localizes at the DNA entry pole where it binds and protects internalized ssDNA. The 2.8-A resolution structure of SsbB bound to ssDNA reveals a similar overall protein architecture and ssDNA-binding surface to that of Escherichia coli SSB. SsbA, which binds ssDNA with higher affinity than SsbB, co-assembles onto SsbB-coated ssDNA and the two proteins inhibit ssDNA binding by the recombinase RecA. During chromosomal transformation, the RecA mediators RecO and DprA provide RecA access to ssDNA. Interestingly, RecO interaction with ssDNA-bound SsbA helps to dislodge both SsbA and SsbB from the DNA more efficiently than if the DNA is coated only with SsbA. Once RecA is nucleated onto the ssDNA, RecA filament elongation displaces SsbA and SsbB and enables RecA-mediated DNA strand exchange. During plasmid transformation, RecO localizes to the entry pole and catalyzes annealing of SsbA- or SsbA/SsbB-coated complementary ssDNAs to form duplex DNA with ssDNA tails. Our results provide a mechanistic framework for rationalizing the coordinated events modulated by SsbA, SsbB and RecO that are crucial for RecA-dependent chromosomal transformation and RecA-independent plasmid transformation. Genetic recombination in Bacillus subtilis: a division of labor between two single-strand DNA-binding proteins.,Yadav T, Carrasco B, Myers AR, George NP, Keck JL, Alonso JC Nucleic Acids Res. 2012 Feb 28. PMID:22373918[2] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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