1hs7: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
[[Image:1hs7.gif|left|200px]] | [[Image:1hs7.gif|left|200px]] | ||
<!-- | |||
The line below this paragraph, containing "STRUCTURE_1hs7", creates the "Structure Box" on the page. | |||
You may change the PDB parameter (which sets the PDB file loaded into the applet) | |||
or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |||
| | or leave the SCENE parameter empty for the default display. | ||
| | --> | ||
{{STRUCTURE_1hs7| PDB=1hs7 | SCENE= }} | |||
}} | |||
'''VAM3P N-TERMINAL DOMAIN SOLUTION STRUCTURE''' | '''VAM3P N-TERMINAL DOMAIN SOLUTION STRUCTURE''' | ||
| Line 30: | Line 27: | ||
[[Category: Wang, Y.]] | [[Category: Wang, Y.]] | ||
[[Category: Yamaguchi, T.]] | [[Category: Yamaguchi, T.]] | ||
[[Category: | [[Category: Up-and-down three-helix bundle insertion preceding proline in an alpha-helix]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 19:10:52 2008'' | |||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | |||
Revision as of 19:10, 2 May 2008
VAM3P N-TERMINAL DOMAIN SOLUTION STRUCTURE
OverviewOverview
Syntaxins and Sec1/munc18 proteins are central to intracellular membrane fusion. All syntaxins comprise a variable N-terminal region, a conserved SNARE motif that is critical for SNARE complex formation, and a transmembrane region. The N-terminal region of neuronal syntaxin 1A contains a three-helix domain that folds back onto the SNARE motif forming a 'closed' conformation; this conformation is required for munc18-1 binding. We have examined the generality of the structural properties of syntaxins by NMR analysis of Vam3p, a yeast syntaxin essential for vacuolar fusion. Surprisingly, Vam3p also has an N-terminal three-helical domain despite lacking apparent sequence homology with syntaxin 1A in this region. However, Vam3p does not form a closed conformation and its N-terminal domain is not required for binding to the Sec1/munc18 protein Vps33p, suggesting that critical distinctions exist in the mechanisms used by syntaxins to govern different types of membrane fusion.
About this StructureAbout this Structure
1HS7 is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.
ReferenceReference
Vam3p structure reveals conserved and divergent properties of syntaxins., Dulubova I, Yamaguchi T, Wang Y, Sudhof TC, Rizo J, Nat Struct Biol. 2001 Mar;8(3):258-64. PMID:11224573 Page seeded by OCA on Fri May 2 19:10:52 2008