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==Se-Met derivative structure of a small alarmone hydrolase (RelH) from Corynebacterium glutamicum== | ==Se-Met derivative structure of a small alarmone hydrolase (RelH) from Corynebacterium glutamicum== | ||
<StructureSection load='7qoc' size='340' side='right'caption='[[7qoc]]' scene=''> | <StructureSection load='7qoc' size='340' side='right'caption='[[7qoc]], [[Resolution|resolution]] 2.30Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7QOC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7QOC FirstGlance]. <br> | <table><tr><td colspan='2'>[[7qoc]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7QOC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7QOC FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7qoc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7qoc OCA], [https://pdbe.org/7qoc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7qoc RCSB], [https://www.ebi.ac.uk/pdbsum/7qoc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7qoc ProSAT]</span></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | ||
<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | |||
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Guanosine-3',5'-bis(diphosphate)_3'-diphosphatase Guanosine-3',5'-bis(diphosphate) 3'-diphosphatase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.7.2 3.1.7.2] </span></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7qoc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7qoc OCA], [https://pdbe.org/7qoc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7qoc RCSB], [https://www.ebi.ac.uk/pdbsum/7qoc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7qoc ProSAT]</span></td></tr> | |||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The bacterial stringent response involves wide-ranging metabolic reprogramming aimed at increasing long-term survivability during stress conditions. One of the hallmarks of the stringent response is the production of a set of modified nucleotides, known as alarmones, which affect a multitude of cellular pathways in diverse ways. Production and degradation of these molecules depend on the activity of enzymes from the RelA/SpoT homologous (RSH) family, which come in both bifunctional (containing domains to both synthesize and hydrolyze alarmones) and monofunctional (consisting of only synthetase or hydrolase domain) variants, of which the structure, activity, and regulation of the bifunctional RSHs have been studied most intensely. Despite playing an important role in guanosine nucleotide homeostasis in particular, mechanisms of regulation of the small alarmone hydrolases (SAH) are still rather unclear. Here, we present crystal structures of SAH enzymes from Corynebacterium glutamicum (RelHCg) and Leptospira levettii (RelHLl) and show that while being highly similar, structural differences in substrate access and dimer conformations might be important for regulating their activity. We propose that a varied dimer form is a general property of the SAH family, based on current structural information as well as prediction models for this class of enzymes. Finally, subtle structural variations between monofunctional and bifunctional enzymes point to how these different classes of enzymes are regulated. | |||
Structural variations between small alarmone hydrolase dimers support different modes of regulation of the stringent response.,Bisiak F, Chrenkova A, Zhang SD, Pedersen JN, Otzen DE, Zhang YE, Brodersen DE J Biol Chem. 2022 Jun 14:102142. doi: 10.1016/j.jbc.2022.102142. PMID:35714769<ref>PMID:35714769</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 7qoc" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Bisiak F]] | [[Category: Bisiak, F]] | ||
[[Category: Brodersen | [[Category: Brodersen, D E]] | ||
[[Category: Chrenkova A]] | [[Category: Chrenkova, A]] | ||
[[Category: Alarmone]] | |||
[[Category: Hd domain]] | |||
[[Category: Hydrolase]] | |||
[[Category: Stress response]] | |||
Revision as of 21:15, 27 July 2022
Se-Met derivative structure of a small alarmone hydrolase (RelH) from Corynebacterium glutamicumSe-Met derivative structure of a small alarmone hydrolase (RelH) from Corynebacterium glutamicum
Structural highlights
Publication Abstract from PubMedThe bacterial stringent response involves wide-ranging metabolic reprogramming aimed at increasing long-term survivability during stress conditions. One of the hallmarks of the stringent response is the production of a set of modified nucleotides, known as alarmones, which affect a multitude of cellular pathways in diverse ways. Production and degradation of these molecules depend on the activity of enzymes from the RelA/SpoT homologous (RSH) family, which come in both bifunctional (containing domains to both synthesize and hydrolyze alarmones) and monofunctional (consisting of only synthetase or hydrolase domain) variants, of which the structure, activity, and regulation of the bifunctional RSHs have been studied most intensely. Despite playing an important role in guanosine nucleotide homeostasis in particular, mechanisms of regulation of the small alarmone hydrolases (SAH) are still rather unclear. Here, we present crystal structures of SAH enzymes from Corynebacterium glutamicum (RelHCg) and Leptospira levettii (RelHLl) and show that while being highly similar, structural differences in substrate access and dimer conformations might be important for regulating their activity. We propose that a varied dimer form is a general property of the SAH family, based on current structural information as well as prediction models for this class of enzymes. Finally, subtle structural variations between monofunctional and bifunctional enzymes point to how these different classes of enzymes are regulated. Structural variations between small alarmone hydrolase dimers support different modes of regulation of the stringent response.,Bisiak F, Chrenkova A, Zhang SD, Pedersen JN, Otzen DE, Zhang YE, Brodersen DE J Biol Chem. 2022 Jun 14:102142. doi: 10.1016/j.jbc.2022.102142. PMID:35714769[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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