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==Crystal structure of M-PMV dUTPASE post-inversion product (dUMP) COMPLEX== | ==Crystal structure of M-PMV dUTPASE post-inversion product (dUMP) COMPLEX== | ||
<StructureSection load='3tq5' size='340' side='right' caption='[[3tq5]], [[Resolution|resolution]] 1.40Å' scene=''> | <StructureSection load='3tq5' size='340' side='right'caption='[[3tq5]], [[Resolution|resolution]] 1.40Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[3tq5]] is a 1 chain structure with sequence from [ | <table><tr><td colspan='2'>[[3tq5]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mpmv Mpmv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TQ5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3TQ5 FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene>, <scene name='pdbligand=UMP:2-DEOXYURIDINE+5-MONOPHOSPHATE'>UMP</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene>, <scene name='pdbligand=UMP:2-DEOXYURIDINE+5-MONOPHOSPHATE'>UMP</scene></td></tr> | ||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2d4n|2d4n]], [[2d4l|2d4l]], [[2d4m|2d4m]], [[3tp1|3tp1]], [[3tpn|3tpn]], [[3tps|3tps]], [[3tpw|3tpw]], [[3tpy|3tpy]], [[3tq3|3tq3]], [[3tq4|3tq4]], [[3trl|3trl]], [[3trn|3trn]], [[3ts6|3ts6]], [[3tsl|3tsl]], [[3tta|3tta]]</td></tr> | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2d4n|2d4n]], [[2d4l|2d4l]], [[2d4m|2d4m]], [[3tp1|3tp1]], [[3tpn|3tpn]], [[3tps|3tps]], [[3tpw|3tpw]], [[3tpy|3tpy]], [[3tq3|3tq3]], [[3tq4|3tq4]], [[3trl|3trl]], [[3trn|3trn]], [[3ts6|3ts6]], [[3tsl|3tsl]], [[3tta|3tta]]</div></td></tr> | ||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">gag-pro ([ | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">gag-pro ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=11855 MPMV])</td></tr> | ||
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[ | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/dUTP_diphosphatase dUTP diphosphatase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.1.23 3.6.1.23] </span></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3tq5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tq5 OCA], [https://pdbe.org/3tq5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3tq5 RCSB], [https://www.ebi.ac.uk/pdbsum/3tq5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3tq5 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
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</div> | </div> | ||
<div class="pdbe-citations 3tq5" style="background-color:#fffaf0;"></div> | <div class="pdbe-citations 3tq5" style="background-color:#fffaf0;"></div> | ||
==See Also== | |||
*[[DUTPase 3D structures|DUTPase 3D structures]] | |||
== References == | == References == | ||
<references/> | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | |||
[[Category: Mpmv]] | [[Category: Mpmv]] | ||
[[Category: DUTP diphosphatase]] | [[Category: DUTP diphosphatase]] | ||
Revision as of 20:08, 6 July 2022
Crystal structure of M-PMV dUTPASE post-inversion product (dUMP) COMPLEXCrystal structure of M-PMV dUTPASE post-inversion product (dUMP) COMPLEX
Structural highlights
Publication Abstract from PubMedDeoxyuridine 5'-triphosphate nucleotidohydrolase from Mason-Pfizer monkey retrovirus (M-PMV dUTPase) is a betaretroviral member of the dUTPase enzyme family. In the mature M-PMV virion, this enzyme is present as the C-terminal domain of the fusion protein nucleocapsid-dUTPase. The homotrimeric organization characteristic of dUTPases is retained in this bifunctional fusion protein. The fusion protein supposedly plays a role in adequate localization of dUTPase activity in the vicinity of nucleic acids during reverse transcription and integration. Here, the nucleocapsid-free dUTPase (48 426 Da) was cocrystallized with a dUTP substrate analogue using the hanging-drop vapour-diffusion method. The obtained crystals belong to the primitive hexagonal space group P6(3), with unit-cell parameters a = 60.6, b = 60.6, c = 63.6 angstroms, alpha = 90, beta = 90, gamma = 120 degrees. Native and PtCl4-derivative data sets were collected using synchrotron radiation to 1.75 and 2.3 angstroms, respectively. Phasing was successfully performed by isomorphous replacement combined with anomalous scattering. Crystallization and preliminary X-ray studies of dUTPase from Mason-Pfizer monkey retrovirus.,Barabas O, Nemeth V, Vertessy BG Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 Apr 1;62(Pt, 4):399-401. Epub 2006 Mar 25. PMID:16582495[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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