1hmh: Difference between revisions

Revision as of 19:00, 2 May 2008

THREE-DIMENSIONAL STRUCTURE OF A HAMMERHEAD RIBOZYME

OverviewOverview

The hammerhead ribozyme is a small catalytic RNA motif made up of three base-paired stems and a core of highly conserved, non-complementary nucleotides essential for catalysis. The X-ray crystallographic structure of a hammerhead RNA-DNA ribozyme-inhibitor complex at 2.6 A resolution reveals that the base-paired stems are A-form helices and that the core has two structural domains. The first domain is formed by the sequence 5'-CUGA following stem I and is a sharp turn identical to the uridine turn of transfer RNA, whereas the second is a non-Watson-Crick three-base-pair duplex with a divalent-ion binding site. The phosphodiester backbone of the DNA inhibitor strand is splayed out at the phosphate 5' to the cleavage site. The structure indicates that the ribozyme may destabilize a substrate strand in order to facilitate twisting of the substrate to allow cleavage of the scissile bond.

About this StructureAbout this Structure

Full crystallographic information is available from OCA.

ReferenceReference

Three-dimensional structure of a hammerhead ribozyme., Pley HW, Flaherty KM, McKay DB, Nature. 1994 Nov 3;372(6501):68-74. PMID:7969422 Page seeded by OCA on Fri May 2 19:00:55 2008

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OCA

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 [[Image:1hmh.jpg|left|200px]]
- {{Structure
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-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1hmh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hmh OCA], [http://www.ebi.ac.uk/pdbsum/1hmh PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1hmh RCSB]</span>
-}}
 '''THREE-DIMENSIONAL STRUCTURE OF A HAMMERHEAD RIBOZYME'''
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 ==About this Structure==
-HMH is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HMH OCA].
+Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HMH OCA].
 ==Reference==
 Three-dimensional structure of a hammerhead ribozyme., Pley HW, Flaherty KM, McKay DB, Nature. 1994 Nov 3;372(6501):68-74. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/7969422 7969422]
-[[Category: Protein complex]]
 [[Category: Flaherty, K M.]]
 [[Category: McKay, D B.]]
 [[Category: Pley, H W.]]
-[[Category: dna-rna hammerhead ribozyme]]
+[[Category: Dna-rna hammerhead ribozyme]]
-[[Category: loop]]
+[[Category: Loop]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 19:00:55 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:07:39 2008''