1hjc: Difference between revisions

Revision as of 18:54, 2 May 2008

CRYSTAL STRUCTURE OF RUNX-1/AML1/CBFALPHA RUNT DOMAIN BOUND TO A DNA FRAGMENT FROM THE CSF-1R PROMOTER

OverviewOverview

The core binding factor (CBF) heterodimeric transcription factors comprised of AML/CBFA/PEBP2alpha/Runx and CBFbeta/PEBP2beta subunits are essential for differentiation of hematopoietic and bone cells, and their mutation is intimately related to the development of acute leukemias and cleidocranial dysplasia. Here, we present the crystal structures of the AML1/Runx-1/CBFalpha(Runt domain)-CBFbeta(core domain)-C/EBPbeta(bZip)-DNA, AML1/Runx-1/CBFalpha(Runt domain)-C/EBPbeta(bZip)-DNA, and AML1/Runx-1/CBFalpha(Runt domain)-DNA complexes. The hydrogen bonding network formed among CBFalpha(Runt domain) and CBFbeta, and CBFalpha(Runt domain) and DNA revealed the allosteric regulation mechanism of CBFalpha(Runt domain)-DNA binding by CBFbeta. The point mutations of CBFalpha related to the aforementioned diseases were also mapped and their effect on DNA binding is discussed.

About this StructureAbout this Structure

1HJC is a Single protein structure of sequence from Mus musculus. Full crystallographic information is available from OCA.

ReferenceReference

Structural analyses of DNA recognition by the AML1/Runx-1 Runt domain and its allosteric control by CBFbeta., Tahirov TH, Inoue-Bungo T, Morii H, Fujikawa A, Sasaki M, Kimura K, Shiina M, Sato K, Kumasaka T, Yamamoto M, Ishii S, Ogata K, Cell. 2001 Mar 9;104(5):755-67. PMID:11257229 Page seeded by OCA on Fri May 2 18:54:16 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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 [[Image:1hjc.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 1hjc |SIZE=350|CAPTION= <scene name='initialview01'>1hjc</scene>, resolution 2.65&Aring;
+The line below this paragraph, containing "STRUCTURE_1hjc", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=DA:2&#39;-DEOXYADENOSINE-5&#39;-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2&#39;-DEOXYCYTIDINE-5&#39;-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2&#39;-DEOXYGUANOSINE-5&#39;-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5&#39;-MONOPHOSPHATE'>DT</scene>
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-|DOMAIN=
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-|RELATEDENTRY=[[1cmo|1CMO]], [[1co1|1CO1]], [[1e50|1E50]], [[1hjb|1HJB]], [[1io4|1IO4]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1hjc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hjc OCA], [http://www.ebi.ac.uk/pdbsum/1hjc PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1hjc RCSB]</span>
-}}
 '''CRYSTAL STRUCTURE OF RUNX-1/AML1/CBFALPHA RUNT DOMAIN BOUND TO A DNA FRAGMENT FROM THE CSF-1R PROMOTER'''
@@ Line 27: / Line 24: @@
 [[Category: Ogata, K.]]
 [[Category: Tahirov, T H.]]
-[[Category: aml]]
+[[Category: Aml]]
-[[Category: aml1]]
+[[Category: Aml1]]
-[[Category: bzip]]
+[[Category: Bzip]]
-[[Category: c/ebp]]
+[[Category: C/ebp]]
-[[Category: cbf]]
+[[Category: Cbf]]
-[[Category: core binding factor]]
+[[Category: Core binding factor]]
-[[Category: oncoprotein]]
+[[Category: Oncoprotein]]
-[[Category: protein-dna complex]]
+[[Category: Protein-dna complex]]
-[[Category: runt]]
+[[Category: Runt]]
-[[Category: runx]]
+[[Category: Runx]]
-[[Category: transcription factor]]
+[[Category: Transcription factor]]
-[[Category: transcription/dna]]
+[[Category: Transcription/dna]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 18:54:16 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:05:59 2008''