3s1l: Difference between revisions
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==Crystal Structure of Apo-form FurX== | ==Crystal Structure of Apo-form FurX== | ||
<StructureSection load='3s1l' size='340' side='right' caption='[[3s1l]], [[Resolution|resolution]] 1.90Å' scene=''> | <StructureSection load='3s1l' size='340' side='right'caption='[[3s1l]], [[Resolution|resolution]] 1.90Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[3s1l]] is a 4 chain structure with sequence from [ | <table><tr><td colspan='2'>[[3s1l]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Cuppj Cuppj]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3S1L OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3S1L FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=P6G:HEXAETHYLENE+GLYCOL'>P6G</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=P6G:HEXAETHYLENE+GLYCOL'>P6G</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | ||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3s2e|3s2e]], [[3s2f|3s2f]], [[3s2g|3s2g]], [[3s2i|3s2i]]</td></tr> | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3s2e|3s2e]], [[3s2f|3s2f]], [[3s2g|3s2g]], [[3s2i|3s2i]]</div></td></tr> | ||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Reut_B3677 ([ | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Reut_B3677 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=264198 CUPPJ])</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3s1l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3s1l OCA], [https://pdbe.org/3s1l PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3s1l RCSB], [https://www.ebi.ac.uk/pdbsum/3s1l PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3s1l ProSAT]</span></td></tr> | ||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
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==See Also== | ==See Also== | ||
*[[Alcohol dehydrogenase|Alcohol dehydrogenase]] | *[[Alcohol dehydrogenase 3D structures|Alcohol dehydrogenase 3D structures]] | ||
== References == | == References == | ||
<references/> | <references/> | ||
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</StructureSection> | </StructureSection> | ||
[[Category: Cuppj]] | [[Category: Cuppj]] | ||
[[Category: Large Structures]] | |||
[[Category: Hayes, R]] | [[Category: Hayes, R]] | ||
[[Category: Hooper, T]] | [[Category: Hooper, T]] | ||
Revision as of 13:34, 22 June 2022
Crystal Structure of Apo-form FurXCrystal Structure of Apo-form FurX
Structural highlights
Publication Abstract from PubMedFurX is a tetrameric Zn-dependent alcohol dehydrogenase (ADH) from Cupriavidus necator JMP134. The enzyme rapidly reduces furfural with NADH as the reducing power. For the first time among characterized ADHs, the high-resolution structures of all reaction steps were obtained in a time-resolved manner, thereby illustrating the complete catalytic events of NADH-dependent reduction of furfural and the dynamic Zn(2+) coordination among Glu66, water, substrate and product. In the fully closed conformation of the NADH complex, the catalytic turnover proved faster than observed for the partially closed conformation due to an effective proton transfer network. The domain motion triggered by NAD(H) association/dissociation appeared to facilitate dynamic interchanges in Zn(2+) coordination with substrate and product molecules, ultimately increasing the enzymatic turnover rate. NAD(+) dissociation appeared to be a slow process, involving multiple steps in concert with a domain opening and reconfiguration of Glu66. This agrees with the report that the cofactor is not dissociated from FurX during ethanol-dependent reduction of furfural, in which ethanol reduces NAD(+) to NADH that is subsequently used for furfural reduction. Furfural reduction mechanism of a zinc-dependent alcohol dehydrogenase from Cupriavidus necator JMP134.,Kang C, Hayes R, Sanchez EJ, Webb BN, Li Q, Hooper T, Nissen MS, Xun L Mol Microbiol. 2012 Jan;83(1):85-95. doi: 10.1111/j.1365-2958.2011.07914.x. Epub , 2011 Nov 20. PMID:22081946[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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