1heu: Difference between revisions

Revision as of 18:46, 2 May 2008

ATOMIC X-RAY STRUCTURE OF LIVER ALCOHOL DEHYDROGENASE CONTAINING CADMIUM AND A HYDROXIDE ADDUCT TO NADH

OverviewOverview

Atomic (1 A) resolution x-ray structures of horse liver alcohol dehydrogenase in complex with NADH revealed the formation of an adduct in the active site between a metal-bound water and NADH. Furthermore, a pronounced distortion of the pyridine ring of NADH was observed. A series of quantum chemical calculations on the water-nicotinamide adduct showed that the puckering of the pyridine ring in the crystal structures can only be reproduced when the water is considered a hydroxide ion. These observations provide fundamental insight into the enzymatic activation of NADH for hydride transfer.

About this StructureAbout this Structure

1HEU is a Single protein structure of sequence from Equus caballus. Full crystallographic information is available from OCA.

ReferenceReference

On the enzymatic activation of NADH., Meijers R, Morris RJ, Adolph HW, Merli A, Lamzin VS, Cedergren-Zeppezauer ES, J Biol Chem. 2001 Mar 23;276(12):9316-21. Epub 2000 Dec 28. PMID:11134046 Page seeded by OCA on Fri May 2 18:46:42 2008

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OCA

@@ Line 1: / Line 1: @@
 [[Image:1heu.jpg|left|200px]]
- {{Structure
+<!--
-|PDB= 1heu |SIZE=350|CAPTION= <scene name='initialview01'>1heu</scene>, resolution 1.15&Aring;
+The line below this paragraph, containing "STRUCTURE_1heu", creates the "Structure Box" on the page.
-|SITE= <scene name='pdbsite=AC1:Cd+Binding+Site+For+Chain+A'>AC1</scene>, <scene name='pdbsite=AC2:Cd+Binding+Site+For+Chain+A'>AC2</scene>, <scene name='pdbsite=AC3:Cd+Binding+Site+For+Chain+B'>AC3</scene>, <scene name='pdbsite=AC4:Cd+Binding+Site+For+Chain+B'>AC4</scene>, <scene name='pdbsite=AC5:Nad+Binding+Site+For+Chain+A'>AC5</scene> and <scene name='pdbsite=AC6:Nad+Binding+Site+For+Chain+B'>AC6</scene>
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=CD:CADMIUM+ION'>CD</scene>, <scene name='pdbligand=MRD:(4R)-2-METHYLPENTANE-2,4-DIOL'>MRD</scene>, <scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Alcohol_dehydrogenase Alcohol dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.1 1.1.1.1] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
-|DOMAIN=
+{{STRUCTURE_1heu|  PDB=1heu  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1heu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1heu OCA], [http://www.ebi.ac.uk/pdbsum/1heu PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1heu RCSB]</span>
-}}
 '''ATOMIC X-RAY STRUCTURE OF LIVER ALCOHOL DEHYDROGENASE CONTAINING CADMIUM AND A HYDROXIDE ADDUCT TO NADH'''
@@ Line 32: / Line 29: @@
 [[Category: Merli, A.]]
 [[Category: Morris, R J.]]
-[[Category: oxidoreductase(nad(a)-choh(d))]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 18:46:42 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:03:52 2008''