1hcj: Difference between revisions

Revision as of 18:42, 2 May 2008

PHOTOPRODUCT OF THE WILD-TYPE AEQUOREA VICTORIA GREEN FLUORESCENT PROTEIN

OverviewOverview

Wild type green fluorescent protein (GFP) from Aequorea victoria absorbs predominantly at 398 nm. Illumination with UV (254 nm) or visible (390 nm) light transforms this state (GFP(398)) into one absorbing at 483 nm (GFP(483)). Here we show that this photoconversion of GFP is a one-photon process that is paralleled by decarboxylation of Glu 222. We propose a mechanism in which decarboxylation is due to electron transfer between the gamma-carboxylate of Glu 222 and the p-hydroxybenzylidene-imidazolidinone chromophore of GFP, followed by reverse transfer of an electron and a proton to the remaining carbon side chain atom of Glu 222. Oxidative decarboxylation of a gamma-carboxylate represents a new type of posttranslational modification that may also occur in enzymes with high-potential reaction intermediates.

About this StructureAbout this Structure

1HCJ is a Single protein structure of sequence from Aequorea victoria. Full crystallographic information is available from OCA.

ReferenceReference

Phototransformation of green fluorescent protein with UV and visible light leads to decarboxylation of glutamate 222., van Thor JJ, Gensch T, Hellingwerf KJ, Johnson LN, Nat Struct Biol. 2002 Jan;9(1):37-41. PMID:11740505 Page seeded by OCA on Fri May 2 18:42:02 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
 [[Image:1hcj.jpg|left|200px]]
- {{Structure
+<!--
-|PDB= 1hcj |SIZE=350|CAPTION= <scene name='initialview01'>1hcj</scene>, resolution 1.80&Aring;
+The line below this paragraph, containing "STRUCTURE_1hcj", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=CSY:[4-(4-HYDROXY-BENZYL)-2-(2-HYDROXY-1-METHYL-ETHYL)-5-OXO-IMIDAZOLIDIN-1-YL]-ACETIC+ACID'>CSY</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY=
+or leave the SCENE parameter empty for the default display.
-|GENE= GFP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6100 Aequorea victoria])
+-->
-|DOMAIN=
+{{STRUCTURE_1hcj|  PDB=1hcj  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1hcj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hcj OCA], [http://www.ebi.ac.uk/pdbsum/1hcj PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1hcj RCSB]</span>
-}}
 '''PHOTOPRODUCT OF THE WILD-TYPE AEQUOREA VICTORIA GREEN FLUORESCENT PROTEIN'''
@@ Line 29: / Line 26: @@
 [[Category: Johnson, L.]]
 [[Category: Thor, J J.Van.]]
-[[Category: beta-barrel]]
+[[Category: Beta-barrel]]
-[[Category: bioluminescence]]
+[[Category: Bioluminescence]]
-[[Category: fluorescent protein]]
+[[Category: Fluorescent protein]]
-[[Category: luminescence]]
+[[Category: Luminescence]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 18:42:02 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:02:18 2008''