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==Crystal Structure of Antitermination Factors NusB and NusE in complex with BoxA RNA== | ==Crystal Structure of Antitermination Factors NusB and NusE in complex with BoxA RNA== | ||
<StructureSection load='3r2c' size='340' side='right' caption='[[3r2c]], [[Resolution|resolution]] 1.90Å' scene=''> | <StructureSection load='3r2c' size='340' side='right'caption='[[3r2c]], [[Resolution|resolution]] 1.90Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[3r2c]] is a 6 chain structure with sequence from [ | <table><tr><td colspan='2'>[[3r2c]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/"aquifex_aeolicus"_huber_and_stetter_2001 "aquifex aeolicus" huber and stetter 2001]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3R2C OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3R2C FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr> | ||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3r2d|3r2d]]</td></tr> | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3r2d|3r2d]]</div></td></tr> | ||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">aq_133, nusB ([ | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">aq_133, nusB ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=63363 "Aquifex aeolicus" Huber and Stetter 2001]), aq_008, NUSE, rpsJ ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=63363 "Aquifex aeolicus" Huber and Stetter 2001])</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3r2c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3r2c OCA], [https://pdbe.org/3r2c PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3r2c RCSB], [https://www.ebi.ac.uk/pdbsum/3r2c PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3r2c ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
[[ | [[https://www.uniprot.org/uniprot/NUSB_AQUAE NUSB_AQUAE]] Involved in the transcription termination process (By similarity). [[https://www.uniprot.org/uniprot/RS10_AQUAE RS10_AQUAE]] Involved in the binding of tRNA to the ribosomes.[HAMAP-Rule:MF_00508] | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
| Line 20: | Line 20: | ||
</div> | </div> | ||
<div class="pdbe-citations 3r2c" style="background-color:#fffaf0;"></div> | <div class="pdbe-citations 3r2c" style="background-color:#fffaf0;"></div> | ||
==See Also== | |||
*[[Ribosomal protein S10|Ribosomal protein S10]] | |||
== References == | == References == | ||
<references/> | <references/> | ||
| Line 25: | Line 28: | ||
</StructureSection> | </StructureSection> | ||
[[Category: Aquifex aeolicus huber and stetter 2001]] | [[Category: Aquifex aeolicus huber and stetter 2001]] | ||
[[Category: Large Structures]] | |||
[[Category: Ji, X]] | [[Category: Ji, X]] | ||
[[Category: Stagno, J R]] | [[Category: Stagno, J R]] | ||
Revision as of 08:46, 15 June 2022
Crystal Structure of Antitermination Factors NusB and NusE in complex with BoxA RNACrystal Structure of Antitermination Factors NusB and NusE in complex with BoxA RNA
Structural highlights
Function[NUSB_AQUAE] Involved in the transcription termination process (By similarity). [RS10_AQUAE] Involved in the binding of tRNA to the ribosomes.[HAMAP-Rule:MF_00508] Publication Abstract from PubMedProcessive transcription antitermination requires the assembly of the complete antitermination complex, which is initiated by the formation of the ternary NusB-NusE-BoxA RNA complex. We have elucidated the crystal structure of this complex, demonstrating that the BoxA RNA is composed of 8 nt that are recognized by the NusB-NusE heterodimer. Functional biologic and biophysical data support the structural observations and establish the relative significance of key protein-protein and protein-RNA interactions. Further crystallographic investigation of a NusB-NusE-dsRNA complex reveals a heretofore unobserved dsRNA binding site contiguous with the BoxA binding site. We propose that the observed dsRNA represents BoxB RNA, as both single-stranded BoxA and double-stranded BoxB components are present in the classical lambda antitermination site. Combining these data with known interactions amongst antitermination factors suggests a specific model for the assembly of the complete antitermination complex. Structural basis for RNA recognition by NusB and NusE in the initiation of transcription antitermination.,Stagno JR, Altieri AS, Bubunenko M, Tarasov SG, Li J, Court DL, Byrd RA, Ji X Nucleic Acids Res. 2011 Jun 7. PMID:21652641[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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