1h99: Difference between revisions

Revision as of 18:35, 2 May 2008

PRD OF LICT ANTITERMINATOR FROM BACILLUS SUBTILIS

OverviewOverview

The transcriptional antiterminator protein LicT regulates the expression of Bacillus subtilis operons involved in beta-glucoside metabolism. It belongs to a newly characterized family of bacterial regulators whose activity is controlled by the phosphoenolpyruvate:sugar phosphotransferase system (PTS). LicT contains an N-terminal RNA-binding domain (56 residues), and a PTS regulation domain (PRD, 221 residues) that is phosphorylated on conserved histidines in response to substrate availability. Replacement of both His207 and His269 with a negatively charged residue (aspartic acid) led to a highly active LicT variant that no longer responds to either induction or catabolite repression signals from the PTS. In contrast to wild type, the activated mutant form of the LicT regulatory domain crystallized easily and provided the first structure of a PRD, determined at 1.55 A resolution. The structure is a homodimer, each monomer containing two analogous alpha-helical domains. The phosphorylation sites are totally buried at the dimer interface and hence inaccessible to phosphorylating partners. The structure suggests important tertiary and quaternary rearrangements upon LicT activation, which could be communicated from the protein C-terminal end up to the RNA-binding domain.

About this StructureAbout this Structure

1H99 is a Single protein structure of sequence from Bacillus subtilis. Full crystallographic information is available from OCA.

ReferenceReference

Crystal structure of an activated form of the PTS regulation domain from the LicT transcriptional antiterminator., van Tilbeurgh H, Le Coq D, Declerck N, EMBO J. 2001 Jul 16;20(14):3789-99. PMID:11447120 Page seeded by OCA on Fri May 2 18:35:40 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
 [[Image:1h99.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 1h99 |SIZE=350|CAPTION= <scene name='initialview01'>1h99</scene>, resolution 1.55&Aring;
+The line below this paragraph, containing "STRUCTURE_1h99", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND=
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY=
+or leave the SCENE parameter empty for the default display.
-|GENE= LICT ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 Bacillus subtilis])
+-->
-|DOMAIN=
+{{STRUCTURE_1h99|  PDB=1h99  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1h99 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1h99 OCA], [http://www.ebi.ac.uk/pdbsum/1h99 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1h99 RCSB]</span>
-}}
 '''PRD OF LICT ANTITERMINATOR FROM BACILLUS SUBTILIS'''
@@ Line 27: / Line 24: @@
 [[Category: Declerck, N.]]
 [[Category: Tilbeurgh, H Van.]]
-[[Category: pts regulatory domain]]
+[[Category: Pts regulatory domain]]
-[[Category: transcriptional antiterminator]]
+[[Category: Transcriptional antiterminator]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 18:35:40 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:00:24 2008''