1h3h: Difference between revisions

Revision as of 18:23, 2 May 2008

STRUCTURAL BASIS FOR SPECIFIC RECOGNITION OF AN RXXK-CONTAINING SLP-76 PEPTIDE BY THE GADS C-TERMINAL SH3 DOMAIN

OverviewOverview

The SH3 domain, which normally recognizes proline-rich sequences, has the potential to bind motifs with an RxxK consensus. To explore this novel specificity, we have determined the solution structure of the Gads T cell adaptor C-terminal SH3 domain in complex with an RSTK-containing peptide, representing its physiological binding site on the SLP-76 docking protein. The SLP-76 peptide engages four distinct binding pockets on the surface of the Gads SH3 domain and upon binding adopts a unique structure characterized by a right-handed 3(10) helix at the RSTK locus, in contrast to the left-handed polyproline type II helix formed by canonical proline-rich SH3 ligands. The structure, and supporting mutagenesis and peptide binding data, reveal a novel mode of ligand recognition by SH3 domains.

About this StructureAbout this Structure

1H3H is a Protein complex structure of sequences from Homo sapiens and Mus musculus. Full crystallographic information is available from OCA.

ReferenceReference

Structural basis for specific binding of the Gads SH3 domain to an RxxK motif-containing SLP-76 peptide: a novel mode of peptide recognition., Liu Q, Berry D, Nash P, Pawson T, McGlade CJ, Li SS, Mol Cell. 2003 Feb;11(2):471-81. PMID:12620234 Page seeded by OCA on Fri May 2 18:23:09 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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 [[Image:1h3h.jpg|left|200px]]
- {{Structure
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 '''STRUCTURAL BASIS FOR SPECIFIC RECOGNITION OF AN RXXK-CONTAINING SLP-76 PEPTIDE BY THE GADS C-TERMINAL SH3 DOMAIN'''
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 [[Category: Nash, P.]]
 [[Category: Pawson, T.]]
-[[Category: sh2 domain]]
+[[Category: Sh2 domain]]
-[[Category: sh3 domain]]
+[[Category: Sh3 domain]]
-[[Category: t-cell signaling]]
+[[Category: T-cell signaling]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 18:23:09 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 20:56:58 2008''