2bnw: Difference between revisions
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<StructureSection load='2bnw' size='340' side='right'caption='[[2bnw]], [[Resolution|resolution]] 2.45Å' scene=''> | <StructureSection load='2bnw' size='340' side='right'caption='[[2bnw]], [[Resolution|resolution]] 2.45Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[2bnw]] is a 8 chain structure with sequence from [ | <table><tr><td colspan='2'>[[2bnw]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/"micrococcus_scarlatinae"_klein_1884 "micrococcus scarlatinae" klein 1884]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BNW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2BNW FirstGlance]. <br> | ||
</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1irq|1irq]], [[2bnz|2bnz]], [[2cax|2cax]]</td></tr> | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1irq|1irq]], [[2bnz|2bnz]], [[2cax|2cax]]</div></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2bnw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bnw OCA], [https://pdbe.org/2bnw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2bnw RCSB], [https://www.ebi.ac.uk/pdbsum/2bnw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2bnw ProSAT]</span></td></tr> | ||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
Revision as of 16:20, 4 May 2022
Structural basis for cooperative binding of Ribbon-Helix-Helix Omega repressor to direct DNA heptad repeatsStructural basis for cooperative binding of Ribbon-Helix-Helix Omega repressor to direct DNA heptad repeats
Structural highlights
Publication Abstract from PubMedRepressor omega regulates transcription of genes required for copy number control, accurate segregation and stable maintenance of inc18 plasmids hosted by Gram-positive bacteria. omega belongs to homodimeric ribbon-helix-helix (RHH2) repressors typified by a central, antiparallel beta-sheet for DNA major groove binding. Homodimeric omega2 binds cooperatively to promotors with 7 to 10 consecutive non-palindromic DNA heptad repeats (5'-(A)/(T)ATCAC(A)/(T)-3', symbolized by -->) in palindromic inverted, converging (--><--) or diverging (<---->) orientation and also, unique to omega2 and contrasting other RHH2 repressors, to non-palindromic direct (-->-->) repeats. Here we investigate with crystal structures how omega2 binds specifically to heptads in minimal operators with (-->-->) and (--><--) repeats. Since the pseudo-2-fold axis relating the monomers in omega(2) passes the central C-G base pair of each heptad with approximately 0.3 A downstream offset, the separation between the pseudo-2-fold axes is exactly 7 bp in (-->-->), approximately 0.6 A shorter in (--><--) but would be approximately 0.6 A longer in (<---->). These variations grade interactions between adjacent omega2 and explain modulations in cooperative binding affinity of omega2 to operators with different heptad orientations. Structures of omega repressors bound to direct and inverted DNA repeats explain modulation of transcription.,Weihofen WA, Cicek A, Pratto F, Alonso JC, Saenger W Nucleic Acids Res. 2006 Mar 9;34(5):1450-8. Print 2006. PMID:16528102[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References |
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