1gxq: Difference between revisions

Revision as of 18:09, 2 May 2008

CRYSTAL STRUCTURE OF THE PHOB EFFECTOR DOMAIN

OverviewOverview

PhoB is a signal transduction response regulator that activates nearly 40 genes in phosphate depletion conditions in E. coli and closely related bacteria. The structure of the PhoB effector domain in complex with its target DNA sequence, or pho box, reveals a novel tandem arrangement in which several monomers bind head to tail to successive 11-base pair direct-repeat sequences, coating one face of a smoothly bent double helix. The protein has a winged helix fold in which the DNA recognition elements comprise helix alpha 3, penetrating the major groove, and a beta hairpin wing interacting with a compressed minor groove via Arg219, tightly sandwiched between the DNA sugar backbones. The transactivation loops protrude laterally in an appropriate orientation to interact with the RNA polymerase sigma(70) subunit, which triggers transcription initiation.

About this StructureAbout this Structure

1GXQ is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

ReferenceReference

Tandem DNA recognition by PhoB, a two-component signal transduction transcriptional activator., Blanco AG, Sola M, Gomis-Ruth FX, Coll M, Structure. 2002 May;10(5):701-13. PMID:12015152 Page seeded by OCA on Fri May 2 18:09:16 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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 [[Image:1gxq.gif|left|200px]]
- {{Structure
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-}}
 '''CRYSTAL STRUCTURE OF THE PHOB EFFECTOR DOMAIN'''
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 [[Category: Gomis-Ruth, F X.]]
 [[Category: Sola, M.]]
-[[Category: activator]]
+[[Category: Activator]]
-[[Category: dna binding]]
+[[Category: Dna binding]]
-[[Category: helix-winged-helix]]
+[[Category: Helix-winged-helix]]
-[[Category: phosphorylation]]
+[[Category: Phosphorylation]]
-[[Category: sensory transduction]]
+[[Category: Sensory transduction]]
-[[Category: transcriptional activator]]
+[[Category: Transcriptional activator]]
-[[Category: two-component signal transduction]]
+[[Category: Two-component signal transduction]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 18:09:16 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 20:53:29 2008''