User:Kia Yang/sandbox: Difference between revisions

Line 1:

This page is being worked on during the Spring 2022 semester.

Methionine is an essential amino acid required by our bodies for healthy cell and tissue growth. Methionine is not naturally derived, and is obtained from our diet in the form of homocysteine. '''Methionine synthase''' (abbrev. MS; EC: 2.1.1.13), a B12-dependent enzyme, is a critical part of the one-carbon metabolism cycle. It catalyzes the methylation of homocysteine to methionine. MS mutations and B-12 deficiencies are associated with serious health conditions such as birth abnormalities and anemia.

==Function==

Methionine is an essential amino acid required by our bodies for healthy cell and tissue growth. It is essential because is not naturally derived, and must be obtained from our diet first in the form of homocysteine. Methionine synthase (abbrev. MS; EC: 2.1.1.13), a B12-dependent enzyme, is a critical part of the one-carbon metabolism cycle because it converts homocysteine to methionine.

[[Image:Overall.jpeg]]

Line 11:

Line 12:

== Relevance ==

~~Methionine deficiency~~ can result in diseases ~~such as birth abnormalities~~<ref name="Kung et al"/>.

MS mutations and B-12 deficiencies can result in diseases<ref name="Kung et al"/>.

== Structural highlights ==

~~== Structural highlights ==~~

The full structure of MS has yet to be determined but studies have found it contains <scene name='90/907471/Superposition_1/2'>4 domains</scene>, each domain with a unique function that bind to Cob(I)alamin as the methyl carrier (in pink), methyltetrahydrofolate as the methyl donor in the catalytic cycle (in blue), Homocysteine as the methyl acceptor (in yellow), and S-adenosylmethionine or SAM (in red) as the methyl donor in the reactivation cycle<ref name="Bandarian et al">DOI: 10.1038/nsb738</ref>. During each cycle, the domains must be positioned close enough to the Cobalamin in order for methyl transfer to be successful.

The full structure of MS has yet to be determined but studies have found it contains <scene name='90/907471/Superposition_1/2'>4 domains</scene>, each domain with a unique function that bind to Cob(I)alamin as the methyl carrier (in pink), methyltetrahydrofolate as the methyl donor in the catalytic cycle (in blue), Homocysteine as the methyl acceptor (in yellow), and S-~~adenosyl-L-methionine~~ or SAM (in red) as the methyl donor in the reactivation cycle<ref name="Bandarian et al">DOI: 10.1038/nsb738</ref>. During each cycle, the domains must be positioned close enough to the Cobalamin in order for methyl transfer to be successful.

== Vitamin B12 ==

@@ Line 1: / Line 1: @@
 This page is being worked on during the Spring 2022 semester.
+Methionine is an essential amino acid required by our bodies for healthy cell and tissue growth. Methionine is not naturally derived, and is obtained from our diet in the form of homocysteine. '''Methionine synthase''' (abbrev. MS; EC: 2.1.1.13), a B12-dependent enzyme, is a critical part of the one-carbon metabolism cycle. It catalyzes the methylation of homocysteine to methionine. MS mutations and B-12 deficiencies are associated with serious health conditions such as birth abnormalities and anemia.
 ==Function==
-Methionine is an essential amino acid required by our bodies for healthy cell and tissue growth. It is essential because is not naturally derived, and must be obtained from our diet first in the form of homocysteine. Methionine synthase (abbrev. MS; EC: 2.1.1.13), a B12-dependent enzyme, is a critical part of the one-carbon metabolism cycle because it converts homocysteine to methionine.
 [[Image:Overall.jpeg]]
@@ Line 11: / Line 12: @@
 == Relevance ==
-Methionine deficiency can result in diseases such as birth abnormalities<ref name="Kung et al"/>.
+MS mutations and B-12 deficiencies can result in diseases<ref name="Kung et al"/>.
+== Structural highlights ==
 <StructureSection load='1k7y' size='310' side='right' caption='B12 dependent fragment of E. coli methionine synthase with Cobalt (in pink)' scene=''>
-== Structural highlights ==
+The full structure of MS has yet to be determined but studies have found it contains <scene name='90/907471/Superposition_1/2'>4 domains</scene>, each domain with a unique function that bind to Cob(I)alamin as the methyl carrier (in pink), methyltetrahydrofolate as the methyl donor in the catalytic cycle (in blue), Homocysteine as the methyl acceptor (in yellow), and S-adenosylmethionine or SAM (in red) as the methyl donor in the reactivation cycle<ref name="Bandarian et al">DOI: 10.1038/nsb738</ref>. During each cycle, the domains must be positioned close enough to the Cobalamin in order for methyl transfer to be successful.
-The full structure of MS has yet to be determined but studies have found it contains <scene name='90/907471/Superposition_1/2'>4 domains</scene>, each domain with a unique function that bind to Cob(I)alamin as the methyl carrier (in pink), methyltetrahydrofolate as the methyl donor in the catalytic cycle (in blue), Homocysteine as the methyl acceptor (in yellow), and S-adenosyl-L-methionine or SAM (in red) as the methyl donor in the reactivation cycle<ref name="Bandarian et al">DOI: 10.1038/nsb738</ref>. During each cycle, the domains must be positioned close enough to the Cobalamin in order for methyl transfer to be successful.
 == Vitamin B12 ==