2wgz: Difference between revisions
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<StructureSection load='2wgz' size='340' side='right'caption='[[2wgz]], [[Resolution|resolution]] 2.12Å' scene=''> | <StructureSection load='2wgz' size='340' side='right'caption='[[2wgz]], [[Resolution|resolution]] 2.12Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[2wgz]] is a 2 chain structure with sequence from [ | <table><tr><td colspan='2'>[[2wgz]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bovin Bovin]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WGZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2WGZ FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=147:1-O-[P-NITROPHENYL]-BETA-D-GALACTOPYRANOSE'>147</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=NPO:P-NITROPHENOL'>NPO</scene>, <scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=147:1-O-[P-NITROPHENYL]-BETA-D-GALACTOPYRANOSE'>147</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=NPO:P-NITROPHENOL'>NPO</scene>, <scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene></td></tr> | ||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2vs4|2vs4]], [[2vxl|2vxl]], [[1vzt|1vzt]], [[2vxm|2vxm]], [[1gx0|1gx0]], [[1vzx|1vzx]], [[1o7q|1o7q]], [[2vs5|2vs5]], [[1gx4|1gx4]], [[2jck|2jck]], [[2jcj|2jcj]], [[2vfz|2vfz]], [[1g93|1g93]], [[1k4v|1k4v]], [[1g8o|1g8o]], [[1gwv|1gwv]], [[1vzu|1vzu]], [[2vs3|2vs3]], [[1o7o|1o7o]], [[2jcl|2jcl]], [[2jco|2jco]], [[1gww|1gww]], [[1fg5|1fg5]]</td></tr> | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2vs4|2vs4]], [[2vxl|2vxl]], [[1vzt|1vzt]], [[2vxm|2vxm]], [[1gx0|1gx0]], [[1vzx|1vzx]], [[1o7q|1o7q]], [[2vs5|2vs5]], [[1gx4|1gx4]], [[2jck|2jck]], [[2jcj|2jcj]], [[2vfz|2vfz]], [[1g93|1g93]], [[1k4v|1k4v]], [[1g8o|1g8o]], [[1gwv|1gwv]], [[1vzu|1vzu]], [[2vs3|2vs3]], [[1o7o|1o7o]], [[2jcl|2jcl]], [[2jco|2jco]], [[1gww|1gww]], [[1fg5|1fg5]]</div></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2wgz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wgz OCA], [https://pdbe.org/2wgz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2wgz RCSB], [https://www.ebi.ac.uk/pdbsum/2wgz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2wgz ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
[[ | [[https://www.uniprot.org/uniprot/GGTA1_BOVIN GGTA1_BOVIN]] Transfer of galactose from UDP-galactose to an acceptor molecule (R). | ||
== Evolutionary Conservation == | == Evolutionary Conservation == | ||
[[Image:Consurf_key_small.gif|200px|right]] | [[Image:Consurf_key_small.gif|200px|right]] | ||
Revision as of 14:07, 6 April 2022
Crystal structure of alpha-1,3 galactosyltransferase (alpha3GT) in a complex with p-nitrophenyl-beta-galactoside (pNP-beta-Gal)Crystal structure of alpha-1,3 galactosyltransferase (alpha3GT) in a complex with p-nitrophenyl-beta-galactoside (pNP-beta-Gal)
Structural highlights
Function[GGTA1_BOVIN] Transfer of galactose from UDP-galactose to an acceptor molecule (R). Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMedThe specificities of glycosyltransferases make them useful for the synthesis of biologically active oligosaccharides, but also restrict their range of products. In substrate engineering, substrate promiscuity is enhanced by attaching removable interactive groups to weak substrates. Thus, the attachment of betap-nitrophenyl converts galactose from a poor into a good substrate of alpha-1,3-galactosyltransferase. The crystallographic structure of a complex of alpha3GT containing p-nitrophenyl-beta-galactoside shows that the p-nitrophenyl binds similarly to the N-acetylglucosamine of the substrate, N-acetyllactosamine, interacting with the indole of Trp249. p-Nitrophenyl, unlike N-acetylglucosamine, makes no H-bonds but has more non-polar interactions, making it an effective monosaccharide mimetic. Crystal structure of alpha-1,3-galactosyltransferase (alpha3GT) in a complex with p-nitrophenyl-beta-galactoside (pNPbetaGal).,Jamaluddin H, Tumbale P, Ferns TA, Thiyagarajan N, Brew K, Acharya KR Biochem Biophys Res Commun. 2009 Aug 7;385(4):601-4. Epub 2009 May 30. PMID:19486884[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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