1gsp: Difference between revisions

Revision as of 17:57, 2 May 2008

RIBONUCLEASE T1 COMPLEXED WITH 2',3'-CGPS, 1 DAY

OverviewOverview

Here we present a time-resolved crystallographic analysis of the hydrolysis of exo (Sp) guanosine 2',3'-cyclophosphorothioate by RNase T1. The use of a slow substrate and fast crystallization methods made it possible to perform the study with conventional data-collection techniques. The results support the idea that the hydrolysis reaction proceeds through a mechanism that is the inverse of the transesterification reaction. In addition, the structures provide an explanation for the differential behavior of RNase T1 towards exo- and endo-cyclic thiophosphates.

About this StructureAbout this Structure

1GSP is a Single protein structure of sequence from Aspergillus oryzae. Full crystallographic information is available from OCA.

ReferenceReference

Hydrolysis of a slow cyclic thiophosphate substrate of RNase T1 analyzed by time-resolved crystallography., Zegers I, Loris R, Dehollander G, Fattah Haikal A, Poortmans F, Steyaert J, Wyns L, Nat Struct Biol. 1998 Apr;5(4):280-3. PMID:9546218 Page seeded by OCA on Fri May 2 17:57:43 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
 [[Image:1gsp.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 1gsp |SIZE=350|CAPTION= <scene name='initialview01'>1gsp</scene>, resolution 2.20&Aring;
+The line below this paragraph, containing "STRUCTURE_1gsp", creates the "Structure Box" on the page.
-|SITE= <scene name='pdbsite=BI1:Site'>BI1</scene>, <scene name='pdbsite=CAL:Site'>CAL</scene> and <scene name='pdbsite=CAT:Active+Site'>CAT</scene>
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=SGP:GUANOSINE-2&#39;,3&#39;-CYCLOPHOSPHOROTHIOATE'>SGP</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Ribonuclease_T(1) Ribonuclease T(1)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.3 3.1.27.3] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
-|DOMAIN=
+{{STRUCTURE_1gsp|  PDB=1gsp  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1gsp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gsp OCA], [http://www.ebi.ac.uk/pdbsum/1gsp PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1gsp RCSB]</span>
-}}
 '''RIBONUCLEASE T1 COMPLEXED WITH 2',3'-CGPS, 1 DAY'''
@@ Line 24: / Line 21: @@
 Hydrolysis of a slow cyclic thiophosphate substrate of RNase T1 analyzed by time-resolved crystallography., Zegers I, Loris R, Dehollander G, Fattah Haikal A, Poortmans F, Steyaert J, Wyns L, Nat Struct Biol. 1998 Apr;5(4):280-3. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9546218 9546218]
 [[Category: Aspergillus oryzae]]
-[[Category: Ribonuclease T(1)]]
 [[Category: Single protein]]
 [[Category: Wyns, L.]]
 [[Category: Zegers, I.]]
-[[Category: endoribonuclease]]
+[[Category: Endoribonuclease]]
-[[Category: hydrolase]]
+[[Category: Hydrolase]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 17:57:43 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 20:50:28 2008''