2nlu: Difference between revisions
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<StructureSection load='2nlu' size='340' side='right'caption='[[2nlu]], [[NMR_Ensembles_of_Models | 16 NMR models]]' scene=''> | <StructureSection load='2nlu' size='340' side='right'caption='[[2nlu]], [[NMR_Ensembles_of_Models | 16 NMR models]]' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[2nlu]] is a 2 chain structure with sequence from [ | <table><tr><td colspan='2'>[[2nlu]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NLU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2NLU FirstGlance]. <br> | ||
</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1ri0|1ri0]], [[2b8a|2b8a]], [[1n27|1n27]]</td></tr> | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1ri0|1ri0]], [[2b8a|2b8a]], [[1n27|1n27]]</div></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2nlu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2nlu OCA], [https://pdbe.org/2nlu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2nlu RCSB], [https://www.ebi.ac.uk/pdbsum/2nlu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2nlu ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
[[ | [[https://www.uniprot.org/uniprot/HDGF_HUMAN HDGF_HUMAN]] Heparin-binding protein, with mitogenic activity for fibroblasts. Acts as a transcriptional repressor.<ref>PMID:17974029</ref> | ||
== Evolutionary Conservation == | == Evolutionary Conservation == | ||
[[Image:Consurf_key_small.gif|200px|right]] | [[Image:Consurf_key_small.gif|200px|right]] | ||
Revision as of 12:06, 23 February 2022
Domain-Swapped Dimer of the PWWP Module of Human Hepatoma-derived Growth FactorDomain-Swapped Dimer of the PWWP Module of Human Hepatoma-derived Growth Factor
Structural highlights
Function[HDGF_HUMAN] Heparin-binding protein, with mitogenic activity for fibroblasts. Acts as a transcriptional repressor.[1] Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMedHepatoma-derived growth factor (hHDGF)-related proteins (HRPs) comprise a new growth factor family sharing a highly conserved and ordered N-terminal PWWP module (residues 1-100, previously referred to as a HATH domain) and a variable disordered C-terminal domain. We have shown that the PWWP module is responsible for heparin binding and have solved its structure in solution. Here, we show that under physiological conditions, both the PWWP module and hHDGF can form dimers. Surface plasmon resonance (SPR) studies revealed that the PWWP dimer binds to heparin with affinity that is two orders of magnitude higher (K(d)=13 nM) than that of the monomeric PWWP module (K(d)=1.2 microM). The monomer-dimer equilibrium properties and NMR structural data together suggest that the PWWP dimer is formed through a domain-swapping mechanism. The domain-swapped PWWP dimer structures were calculated on the basis of the NMR data. The results show that the two PWWP protomers exchange their N-terminal hairpin to form a domain-swapped dimer. The two monomers in a dimer are linked by the long flexible L2 loops, a feature supported by NMR relaxation data for the monomer and dimer. The enhanced heparin-binding affinity of the dimer can be rationalized in the framework of the dimer structure. PWWP module of human hepatoma-derived growth factor forms a domain-swapped dimer with much higher affinity for heparin.,Sue SC, Lee WT, Tien SC, Lee SC, Yu JG, Wu WJ, Wu WG, Huang TH J Mol Biol. 2007 Mar 23;367(2):456-72. Epub 2007 Jan 9. PMID:17270212[2] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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