7ck4: Difference between revisions
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ck4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ck4 OCA], [https://pdbe.org/7ck4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ck4 RCSB], [https://www.ebi.ac.uk/pdbsum/7ck4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ck4 ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ck4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ck4 OCA], [https://pdbe.org/7ck4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ck4 RCSB], [https://www.ebi.ac.uk/pdbsum/7ck4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ck4 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The structures of a cyanophage small heat shock protein (sHSP) were determined as octahedrons of 24-mers and 48-mers and as icosahedrons of 60-mers. An N-terminal deletion construct of an 18 kDa sHSP of Synechococcus sp. phage S-ShM2 crystallized as a 24-mer and its structure was determined at a resolution of 7 A. The negative stain electron microscopy (EM) images showed that the full-length protein is a mixture of a major population of larger and a minor population of smaller cage-like particles. Their structures have been determined by electron cryomicroscopy 3D image reconstruction at a resolution of 8 A. The larger particles are 60-mers with icosahedral symmetry and the smaller ones are 48-mers with octahedral symmetry. These structures are the first of the viral/phage origin and the 60-mer is the largest and the first icosahedral assembly to be reported for sHSPs. | |||
Multiple nanocages of a cyanophage small heat shock protein with icosahedral and octahedral symmetries.,Biswas S, Garg P, Dutta S, Suguna K Sci Rep. 2021 Oct 25;11(1):21023. doi: 10.1038/s41598-021-00172-2. PMID:34697325<ref>PMID:34697325</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 7ck4" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[Heat Shock Protein structures|Heat Shock Protein structures]] | |||
== References == | |||
<references/> | |||
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</StructureSection> | </StructureSection> | ||
Revision as of 10:31, 9 February 2022
Structural and functional analysis of small heat shock protein from Synechococcus phage S-ShM2Structural and functional analysis of small heat shock protein from Synechococcus phage S-ShM2
Structural highlights
Publication Abstract from PubMedThe structures of a cyanophage small heat shock protein (sHSP) were determined as octahedrons of 24-mers and 48-mers and as icosahedrons of 60-mers. An N-terminal deletion construct of an 18 kDa sHSP of Synechococcus sp. phage S-ShM2 crystallized as a 24-mer and its structure was determined at a resolution of 7 A. The negative stain electron microscopy (EM) images showed that the full-length protein is a mixture of a major population of larger and a minor population of smaller cage-like particles. Their structures have been determined by electron cryomicroscopy 3D image reconstruction at a resolution of 8 A. The larger particles are 60-mers with icosahedral symmetry and the smaller ones are 48-mers with octahedral symmetry. These structures are the first of the viral/phage origin and the 60-mer is the largest and the first icosahedral assembly to be reported for sHSPs. Multiple nanocages of a cyanophage small heat shock protein with icosahedral and octahedral symmetries.,Biswas S, Garg P, Dutta S, Suguna K Sci Rep. 2021 Oct 25;11(1):21023. doi: 10.1038/s41598-021-00172-2. PMID:34697325[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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