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==Structural Characterization of Beta Cyanoalanine Synthase from Tetranychus Urticae (two-spotted spider mite)== | ==Structural Characterization of Beta Cyanoalanine Synthase from Tetranychus Urticae (two-spotted spider mite)== | ||
<StructureSection load='6xo2' size='340' side='right'caption='[[6xo2]]' scene=''> | <StructureSection load='6xo2' size='340' side='right'caption='[[6xo2]], [[Resolution|resolution]] 1.60Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6XO2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6XO2 FirstGlance]. <br> | <table><tr><td colspan='2'>[[6xo2]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Tetur Tetur]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6XO2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6XO2 FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6xo2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6xo2 OCA], [https://pdbe.org/6xo2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6xo2 RCSB], [https://www.ebi.ac.uk/pdbsum/6xo2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6xo2 ProSAT]</span></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=PLP:PYRIDOXAL-5-PHOSPHATE'>PLP</scene></td></tr> | ||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[6pmu|6pmu]]</div></td></tr> | |||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">107363798 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=32264 TETUR])</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6xo2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6xo2 OCA], [https://pdbe.org/6xo2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6xo2 RCSB], [https://www.ebi.ac.uk/pdbsum/6xo2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6xo2 ProSAT]</span></td></tr> | |||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Tetranychus urticae is a polyphagous spider mite that can feed on more than 1100 plant species including cyanogenic plants. The herbivore genome contains a horizontally acquired gene tetur10g01570 (TuCAS) that was previously shown to participate in cyanide detoxification. To understand the structure and determine the function of TuCAS in T. urticae, crystal structures of the protein with lysine conjugated pyridoxal phosphate (PLP) were determined. These structures reveal extensive TuCAS homology with the beta-substituted alanine synthase family, and they show that this enzyme utilizes a similar chemical mechanism involving a stable alpha-aminoacrylate intermediate in beta-cyanoalanine and cysteine synthesis. We demonstrate that TuCAS is more efficient in the synthesis of beta-cyanoalanine, which is a product of the detoxification reaction between cysteine and cyanide, than in the biosynthesis of cysteine. Also, the enzyme carries additional enzymatic activities that were not previously described. We show that TuCAS can detoxify cyanide using O-acetyl-L-serine as a substrate, leading to the direct formation of beta-cyanoalanine. Moreover, it catalyzes the reaction between the TuCAS-bound alpha-aminoacrylate intermediate and aromatic compounds with a thiol group. In addition, we have tested several compounds as TuCAS inhibitors. Overall, this study identifies additional functions for TuCAS and provides new molecular insight into the xenobiotic metabolism of T. urticae. | |||
Structural and functional characterization of beta-cyanoalanine synthase from Tetranychus urticae.,Daneshian L, Renggli I, Hanaway R, Offermann LR, Schlachter CR, Hernandez Arriaza R, Henry S, Prakash R, Wybouw N, Dermauw W, Shimizu LS, Van Leeuwen T, Makris TM, Grbic V, Grbic M, Chruszcz M Insect Biochem Mol Biol. 2022 Jan 19;142:103722. doi: 10.1016/j.ibmb.2022.103722. PMID:35063675<ref>PMID:35063675</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 6xo2" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Chruszcz M]] | [[Category: Tetur]] | ||
[[Category: Daneshian L]] | [[Category: Chruszcz, M]] | ||
[[Category: Dermauw W]] | [[Category: Daneshian, L]] | ||
[[Category: Schlachter C]] | [[Category: Dermauw, W]] | ||
[[Category: | [[Category: Leeuwen, T Van]] | ||
[[Category: | [[Category: Schlachter, C]] | ||
[[Category: Wybouw, N]] | |||
[[Category: Beta cyanoalanine synthase]] | |||
[[Category: Biosynthetic protein]] | |||
[[Category: Plp. two-spotted spider mite]] | |||