1ga1: Difference between revisions

Revision as of 17:20, 2 May 2008

CRYSTAL STRUCTURE ANALYSIS OF PSCP (PSEUDOMONAS SERINE-CARBOXYL PROTEINASE) COMPLEXED WITH A FRAGMENT OF IODOTYROSTATIN (THIS ENZYME RENAMED "SEDOLISIN" IN 2003)

OverviewOverview

The crystal structure of pepstatin-insensitive carboxyl proteinase (PCP) from Pseudomonas sp. 101, an enzyme with no overall sequence similarity to any other proteinases of known structure, was solved using crystals soaked in sodium bromide solution and then cryocooled. A data set collected at the bromine peak absorption wavelength was sufficient for calculation of an excellent map and the entire process of phasing and tracing the maps required almost no direct human intervention. The process of structure solution using single-wavelength data was compared with three-wavelength multiwavelength anomalous diffraction (MAD); although the latter resulted in slightly better maps, the use of this much more labor-intensive approach did not significantly improve the ability to solve the structure. The successful phasing approaches are compared with several less successful attempts utilizing other crystal forms of the enzyme and the practical aspects of the use of bromine as a heavy-atom derivative are discussed. In conclusion, the use of halides with single-wavelength diffraction data fulfills the requirements of being a first-choice method of high-throughput structure solution for the emerging field of structural genomics.

About this StructureAbout this Structure

1GA1 is a Single protein structure of sequence from Pseudomonas sp.. Full crystallographic information is available from OCA.

ReferenceReference

Practical experience with the use of halides for phasing macromolecular structures: a powerful tool for structural genomics., Dauter Z, Li M, Wlodawer A, Acta Crystallogr D Biol Crystallogr. 2001 Feb;57(Pt 2):239-49. PMID:11173470 Page seeded by OCA on Fri May 2 17:20:04 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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 [[Image:1ga1.gif|left|200px]]
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 '''CRYSTAL STRUCTURE ANALYSIS OF PSCP (PSEUDOMONAS SERINE-CARBOXYL PROTEINASE) COMPLEXED WITH A FRAGMENT OF IODOTYROSTATIN (THIS ENZYME RENAMED "SEDOLISIN" IN 2003)'''
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 [[Category: Li, M.]]
 [[Category: Wlodawer, A.]]
-[[Category: serine-carboxyl proteinase]]
+[[Category: Serine-carboxyl proteinase]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 17:20:04 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 20:39:50 2008''