1g78: Difference between revisions

Revision as of 17:13, 2 May 2008

X-RAY STRUCTURE OF ESCHERICHIA COLI PYRIDOXINE 5'-PHOSPHATE OXIDASE COMPLEXED WITH PYRIDOXAL 5'-PHOSPHATE AT 2.0 A RESOLUTION

OverviewOverview

Escherichia coli pyridoxine 5'-phosphate oxidase catalyzes the terminal step in the biosynthesis of pyridoxal 5'-phosphate by the FMN oxidation of pyridoxine 5'-phosphate forming FMNH(2) and H(2)O(2). Recent studies have shown that in addition to the active site, pyridoxine 5'-phosphate oxidase contains a non-catalytic site that binds pyridoxal 5'-phosphate tightly. The crystal structure of pyridoxine 5'-phosphate oxidase from E. coli with one or two molecules of pyridoxal 5'-phosphate bound to each monomer has been determined to 2.0 A resolution. One of the pyridoxal 5'-phosphate molecules is clearly bound at the active site with the aldehyde at C4' of pyridoxal 5'-phosphate near N5 of the bound FMN. A protein conformational change has occurred that partially closes the active site. The orientation of the bound pyridoxal 5'-phosphate suggests that the enzyme catalyzes a hydride ion transfer between C4' of pyridoxal 5'-phosphate and N5 of FMN. When the crystals are soaked with excess pyridoxal 5'-phosphate an additional molecule of this cofactor is also bound about 11 A from the active site. A possible tunnel exists between the two sites so that pyridoxal 5'-phosphate formed at the active site may transfer to the non-catalytic site without passing though the solvent.

About this StructureAbout this Structure

1G78 is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

ReferenceReference

X-ray structure of Escherichia coli pyridoxine 5'-phosphate oxidase complexed with pyridoxal 5'-phosphate at 2.0 A resolution., Safo MK, Musayev FN, di Salvo ML, Schirch V, J Mol Biol. 2001 Jul 20;310(4):817-26. PMID:11453690 Page seeded by OCA on Fri May 2 17:13:42 2008

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OCA

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 [[Image:1g78.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 1g78 |SIZE=350|CAPTION= <scene name='initialview01'>1g78</scene>, resolution 2.20&Aring;
+The line below this paragraph, containing "STRUCTURE_1g78", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=PLP:PYRIDOXAL-5&#39;-PHOSPHATE'>PLP</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Pyridoxal_5'-phosphate_synthase Pyridoxal 5'-phosphate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.4.3.5 1.4.3.5] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE=
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-|DOMAIN=
+{{STRUCTURE_1g78|  PDB=1g78  |  SCENE=  }}
-|RELATEDENTRY=[[1g76|1G76]], [[1g77|1G77]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1g78 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1g78 OCA], [http://www.ebi.ac.uk/pdbsum/1g78 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1g78 RCSB]</span>
-}}
 '''X-RAY STRUCTURE OF ESCHERICHIA COLI PYRIDOXINE 5'-PHOSPHATE OXIDASE COMPLEXED WITH PYRIDOXAL 5'-PHOSPHATE AT 2.0 A RESOLUTION'''
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 [[Category: Salvo, M L.di.]]
 [[Category: Schirch, V.]]
-[[Category: fmn complex]]
+[[Category: Fmn complex]]
-[[Category: plp complex]]
+[[Category: Plp complex]]
-[[Category: pyridoxine 5'-phosphate]]
+[[Category: Pyridoxine 5'-phosphate]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 17:13:42 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 20:38:02 2008''