1g3m: Difference between revisions

Revision as of 17:05, 2 May 2008

CRYSTAL STRUCTURE OF HUMAN ESTROGEN SULFOTRANSFERASE IN COMPLEX WITH IN-ACTIVE COFACTOR PAP AND 3,5,3',5'-TETRACHLORO-BIPHENYL-4,4'-DIOL

OverviewOverview

Certain hydroxylated polychlorinated biphenyls (OH-PCBs) inhibit the human estrogen sulfotransferase (hEST) at subnanomolar concentrations, suggesting a possible pathway for PCB toxicity due to environmental exposure in humans. To address the structural basis of the inhibition, we have determined the crystal structure of hEST in the presence of the sulfuryl donor product 3 -phosphoadenosine 5 -phosphate and the OH-PCB 4,4 -OH 3,5,3,5 -tetraCB. The OH-PCB binds in the estrogen binding site with the position of the first phenolic ring in an orientation similar to the phenolic ring of 17 beta-estradiol. Interestingly, the OH-PCB does not bind in a planar conformation, but rather with a 30-degree twist between the phenyl rings. The crystal structure of hEST with the OH-PCB bound gives physical evidence that certain OH-PCBs can mimic binding of estrogenic compounds in biological systems.

About this StructureAbout this Structure

1G3M is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

ReferenceReference

Crystallographic analysis of a hydroxylated polychlorinated biphenyl (OH-PCB) bound to the catalytic estrogen binding site of human estrogen sulfotransferase., Shevtsov S, Petrotchenko EV, Pedersen LC, Negishi M, Environ Health Perspect. 2003 Jun;111(7):884-8. PMID:12782487 Page seeded by OCA on Fri May 2 17:05:47 2008

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OCA

@@ Line 1: / Line 1: @@
 [[Image:1g3m.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 1g3m |SIZE=350|CAPTION= <scene name='initialview01'>1g3m</scene>, resolution 1.70&Aring;
+The line below this paragraph, containing "STRUCTURE_1g3m", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=A3P:ADENOSINE-3&#39;-5&#39;-DIPHOSPHATE'>A3P</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=PCQ:3,5,3&#39;,5&#39;-TETRACHLORO-BIPHENYL-4,4&#39;-DIOL'>PCQ</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Estrone_sulfotransferase Estrone sulfotransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.8.2.4 2.8.2.4] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE= STE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])
+-->
-|DOMAIN=
+{{STRUCTURE_1g3m|  PDB=1g3m  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1g3m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1g3m OCA], [http://www.ebi.ac.uk/pdbsum/1g3m PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1g3m RCSB]</span>
-}}
 '''CRYSTAL STRUCTURE OF HUMAN ESTROGEN SULFOTRANSFERASE IN COMPLEX WITH IN-ACTIVE COFACTOR PAP AND 3,5,3',5'-TETRACHLORO-BIPHENYL-4,4'-DIOL'''
@@ Line 30: / Line 27: @@
 [[Category: Petrochenko, E V.]]
 [[Category: Shevtsov, S.]]
-[[Category: estrogen]]
+[[Category: Estrogen]]
-[[Category: human]]
+[[Category: Human]]
-[[Category: pcb]]
+[[Category: Pcb]]
-[[Category: sulfotransferase]]
+[[Category: Sulfotransferase]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 17:05:47 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 20:35:55 2008''