FirstGlance/Evaluating Protein Crosslinks: Difference between revisions
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[[7ns5]] is a homo-tetramer. FirstGlance suggests a possible Lys-Cys NOS crosslink in chain B. The fact that FirstGlance suggests no such crosslinks in the other 3 chains is a | [[7ns5]] is a homo-tetramer. FirstGlance suggests a possible Lys-Cys NOS crosslink in chain B. The fact that FirstGlance suggests no such crosslinks in the other 3 chains is a hint that the proximity of the sidechain nitrogen of Lys167 to the sulfur in Cys165 in chain B is a clash. The atomic model shows an improbable N-S bond between the sidechains. Further, electron density (above left) is missing for the last 3 atoms of the lysine sidechain, consistent with it being misplaced (<span style="color:#00a000;font-weight:bold;">green isomesh</span>). The difference electron density map (above right) reveals that there is no electron density consistent with the lysine sidechain nitrogen being located where it was placed in the model (<span style="color:#b00000;font-weight:bold;">dark red isomesh</span>). In conclusion, this is a clash in chain B, not an NOS crosslink. | ||
==Notes and References== | ==Notes and References== | ||
<references /> | <references /> | ||