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==Crystal structure of Streptomyces tyrosinase in a complex with caddie Y98F mutant soaked in a Cu(II)-containing solution for 80 hr== | ==Crystal structure of Streptomyces tyrosinase in a complex with caddie Y98F mutant soaked in a Cu(II)-containing solution for 80 hr== | ||
<StructureSection load='3ax0' size='340' side='right' caption='[[3ax0]], [[Resolution|resolution]] 1.40Å' scene=''> | <StructureSection load='3ax0' size='340' side='right'caption='[[3ax0]], [[Resolution|resolution]] 1.40Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[3ax0]] is a 2 chain structure with sequence from [ | <table><tr><td colspan='2'>[[3ax0]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/"actinomyces_castaneoglobisporus"_yen "actinomyces castaneoglobisporus" yen]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AX0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3AX0 FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=NO3:NITRATE+ION'>NO3</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=NO3:NITRATE+ION'>NO3</scene></td></tr> | ||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3aws|3aws]], [[3awt|3awt]], [[3awu|3awu]], [[3awv|3awv]], [[3aww|3aww]], [[3awx|3awx]], [[3awy|3awy]], [[3awz|3awz]]</td></tr> | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3aws|3aws]], [[3awt|3awt]], [[3awu|3awu]], [[3awv|3awv]], [[3aww|3aww]], [[3awx|3awx]], [[3awy|3awy]], [[3awz|3awz]]</div></td></tr> | ||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">TYRC ([ | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">TYRC ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=79261 "Actinomyces castaneoglobisporus" Yen]), ORF378 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=79261 "Actinomyces castaneoglobisporus" Yen])</td></tr> | ||
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[ | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Tyrosinase Tyrosinase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.18.1 1.14.18.1] </span></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ax0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ax0 OCA], [https://pdbe.org/3ax0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ax0 RCSB], [https://www.ebi.ac.uk/pdbsum/3ax0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ax0 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
| Line 21: | Line 21: | ||
==See Also== | ==See Also== | ||
*[[Tyrosinase|Tyrosinase]] | *[[Tyrosinase 3D structures|Tyrosinase 3D structures]] | ||
== References == | == References == | ||
<references/> | <references/> | ||
| Line 27: | Line 27: | ||
</StructureSection> | </StructureSection> | ||
[[Category: Actinomyces castaneoglobisporus yen]] | [[Category: Actinomyces castaneoglobisporus yen]] | ||
[[Category: Large Structures]] | |||
[[Category: Tyrosinase]] | [[Category: Tyrosinase]] | ||
[[Category: Matoba, Y]] | [[Category: Matoba, Y]] | ||
Revision as of 18:07, 29 December 2021
Crystal structure of Streptomyces tyrosinase in a complex with caddie Y98F mutant soaked in a Cu(II)-containing solution for 80 hrCrystal structure of Streptomyces tyrosinase in a complex with caddie Y98F mutant soaked in a Cu(II)-containing solution for 80 hr
Structural highlights
Publication Abstract from PubMedThe Cu(II)-soaked crystal structure of tyrosinase that is present in a complex with a protein, designated "caddie," which we previously determined, possesses two copper ions at its catalytic center. We had identified two copper-binding sites in the caddie protein and speculated that copper bound to caddie may be transported to the tyrosinase catalytic center. In our present study, at a 1.16-1.58 A resolution, we determined the crystal structures of tyrosinase complexed with caddie prepared by altering the soaking time of the copper ion and the structures of tyrosinase complexed with different caddie mutants that display little or no capacity to activate tyrosinase. Based on these structures, we propose a molecular mechanism by which two copper ions are transported to the tyrosinase catalytic center with the assistance of caddie acting as a metallochaperone. A molecular mechanism for copper transportation to tyrosinase that is assisted by a metallochaperone, caddie protein.,Matoba Y, Bando N, Oda K, Noda M, Higashikawa F, Kumagai T, Sugiyama M J Biol Chem. 2011 Aug 26;286(34):30219-31. Epub 2011 Jul 5. PMID:21730070[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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