1fxx: Difference between revisions

Revision as of 16:53, 2 May 2008

THE STRUCTURE OF EXONUCLEASE I SUGGESTS HOW PROCESSIVITY IS ACHIEVED

OverviewOverview

Exonuclease I (ExoI) from Escherichia coli is a monomeric enzyme that processively degrades single stranded DNA in the 3' to 5' direction and has been implicated in DNA recombination and repair. Determination of the structure of ExoI to 2.4 A resolution using X-ray crystallography verifies the expected correspondence between a region of ExoI and the exonuclease (or proofreading) domains of the DNA polymerases. The overall fold of ExoI also includes two other regions, one of which extends the exonuclease domain and another that can be described as an elaborated SH3 domain. These three regions combine to form a molecule that is shaped like the letter C, although it also contains a segment that effectively converts the C into an O-like shape. The structure of ExoI thus provides additional support for the idea that DNA metabolizing enzymes achieve processivity by completely enclosing the DNA.

About this StructureAbout this Structure

1FXX is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

ReferenceReference

Structure of Escherichia coli exonuclease I suggests how processivity is achieved., Breyer WA, Matthews BW, Nat Struct Biol. 2000 Dec;7(12):1125-8. PMID:11101894 Page seeded by OCA on Fri May 2 16:53:25 2008

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OCA

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 [[Image:1fxx.jpg|left|200px]]
- {{Structure
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+The line below this paragraph, containing "STRUCTURE_1fxx", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Exodeoxyribonuclease_I Exodeoxyribonuclease I], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.11.1 3.1.11.1] </span>
+or leave the SCENE parameter empty for the default display.
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-|DOMAIN=
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-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1fxx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fxx OCA], [http://www.ebi.ac.uk/pdbsum/1fxx PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1fxx RCSB]</span>
-}}
 '''THE STRUCTURE OF EXONUCLEASE I SUGGESTS HOW PROCESSIVITY IS ACHIEVED'''
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 [[Category: Breyer, W A.]]
 [[Category: Matthews, B W.]]
-[[Category: alpha-beta domain]]
+[[Category: Alpha-beta domain]]
-[[Category: dnaq superfamily]]
+[[Category: Dnaq superfamily]]
-[[Category: sh3-like domain]]
+[[Category: Sh3-like domain]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 16:53:25 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 20:32:30 2008''