1fw8: Difference between revisions

Revision as of 16:50, 2 May 2008

CIRCULARLY PERMUTED PHOSPHOGLYCERATE KINASE FROM YEAST: PGK P72

OverviewOverview

The crystallographic structure of a circularly permuted form of yeast PGK, 72p yPGK, has been determined to a resolution of 2.3 A by molecular replacement. In this engineered protein, the C- and N-terminal residues of the wild-type protein are directly connected by a peptide bond and new N- and C-terminal residues are located within the N-terminal domain. The overall fold of the protein is very similar to that of the wild-type protein, directly demonstrating that the continuity of a folding unit is not relevant to the folding process of the whole protein. Only limited structural changes were observed: these were in the regions associated with the new connection, in a long flexible loop in the permuted domain and in the vicinity of Arg38, a functionally important residue. The relative positions of the two domains suggested that this permuted protein adopts one of the most open/twisted conformations seen amongst PGKs of known structure. The effect of the mutation on the functional properties is more easily accounted for by a restriction of hinge-bending motion than by structural changes in the protein.

About this StructureAbout this Structure

1FW8 is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.

ReferenceReference

Structure of a circularly permuted phosphoglycerate kinase., Tougard P, Bizebard T, Ritco-Vonsovici M, Minard P, Desmadril M, Acta Crystallogr D Biol Crystallogr. 2002 Dec;58(Pt 12):2018-23. Epub 2002, Nov 23. PMID:12454459 Page seeded by OCA on Fri May 2 16:50:10 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
 [[Image:1fw8.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 1fw8 |SIZE=350|CAPTION= <scene name='initialview01'>1fw8</scene>, resolution 2.3&Aring;
+The line below this paragraph, containing "STRUCTURE_1fw8", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Phosphoglycerate_kinase Phosphoglycerate kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.2.3 2.7.2.3] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
-|DOMAIN=
+{{STRUCTURE_1fw8|  PDB=1fw8  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1fw8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fw8 OCA], [http://www.ebi.ac.uk/pdbsum/1fw8 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1fw8 RCSB]</span>
-}}
 '''CIRCULARLY PERMUTED PHOSPHOGLYCERATE KINASE FROM YEAST: PGK P72'''
@@ Line 31: / Line 28: @@
 [[Category: Ritco-Vonsovici, M.]]
 [[Category: Tougard, P.]]
-[[Category: glycolysis]]
+[[Category: Glycolysis]]
-[[Category: kinase]]
+[[Category: Kinase]]
-[[Category: mutant]]
+[[Category: Mutant]]
-[[Category: permutation]]
+[[Category: Permutation]]
-[[Category: permuted sequence]]
+[[Category: Permuted sequence]]
-[[Category: pgk]]
+[[Category: Pgk]]
-[[Category: phosphoglycerate kinase]]
+[[Category: Phosphoglycerate kinase]]
-[[Category: phosphotransferase]]
+[[Category: Phosphotransferase]]
-[[Category: protein folding,two-domain protein]]
+[[Category: Protein folding,two-domain protein]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 16:50:10 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 20:31:26 2008''