1fua: Difference between revisions

Revision as of 16:46, 2 May 2008

L-FUCULOSE 1-PHOSPHATE ALDOLASE CRYSTAL FORM T

OverviewOverview

The structure of the class II zinc-ion dependent L-fuculose-1-phosphate aldolase from Escherichia coli in its tetragonal crystal form has been established at 1.92 A resolution. The homotetrameric enzyme has a molecular mass of 4 x 24 kDa and follows C(4) symmetry. The structure model is exactly symmetrical, which contradicts an observed birefringence anomaly of the crystals. The four catalytic centers are located in deep clefts at the interfaces of adjacent subunits. The zinc ion is coordinated by three histidines and one glutamate in an almost tetrahedral arrangement. In contrast to numerous other catalytically competent zinc ions, there is no water molecule in the ligand sphere. Replacement of zinc by a cobalt ion caused only small structural changes. A search through the Protein Data Bank indicated that the chain fold is novel. Sequence homology searches revealed a significant similarity to the bacterial L-ribulose-5-phosphate 4-epimerase.

About this StructureAbout this Structure

1FUA is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

ReferenceReference

Refined high-resolution structure of the metal-ion dependent L-fuculose-1-phosphate aldolase (class II) from Escherichia coli., Dreyer MK, Schulz GE, Acta Crystallogr D Biol Crystallogr. 1996 Nov 1;52(Pt 6):1082-91. PMID:15299567 Page seeded by OCA on Fri May 2 16:46:31 2008

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OCA

@@ Line 1: / Line 1: @@
 [[Image:1fua.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 1fua |SIZE=350|CAPTION= <scene name='initialview01'>1fua</scene>, resolution 1.92&Aring;
+The line below this paragraph, containing "STRUCTURE_1fua", creates the "Structure Box" on the page.
-|SITE= <scene name='pdbsite=ACT:The+Active+Center+Is+Defined+By+The+Zn+Ion+And+The+Four+...'>ACT</scene> and <scene name='pdbsite=PBS:The+Substrate+Phosphate+Binding+Site+Near+The+Active+Cen+...'>PBS</scene>
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=BME:BETA-MERCAPTOETHANOL'>BME</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/L-fuculose-phosphate_aldolase L-fuculose-phosphate aldolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.2.17 4.1.2.17] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
-|DOMAIN=
+{{STRUCTURE_1fua|  PDB=1fua  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1fua FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fua OCA], [http://www.ebi.ac.uk/pdbsum/1fua PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1fua RCSB]</span>
-}}
 '''L-FUCULOSE 1-PHOSPHATE ALDOLASE CRYSTAL FORM T'''
@@ Line 28: / Line 25: @@
 [[Category: Dreyer, M K.]]
 [[Category: Schulz, G E.]]
-[[Category: class ii aldolase]]
+[[Category: Class ii aldolase]]
-[[Category: lyase (aldehyde)]]
+[[Category: Zinc enzyme]]
-[[Category: zinc enzyme]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 16:46:31 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 20:30:25 2008''