Enkephalin: Difference between revisions
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The main conformations of enkephalin found in crystals have been classified in three categories, described as “extended,” “single bend,” and “double bend.”<ref>Deschamps J. R., George C., Flippen-Anderson J. L. (1996). Structural studies of opioid peptides: a review of recent progress in x-ray diffraction studies. Biopolymers 40, 121–139. 10.1002/bip.360400102</ref>. The picture below represents molecular models of Leu-enkephalin in the three main conformations found in solid state determinations when the peptide is dissolved in a DMSO/water cryomixture at 275 K. <ref>Amodeo P., Naider F., Picone D., Tancredi T., Temussi P. A. (1998). Conformational sampling of bioactive conformers: a low temperature NMR study of 15N-Leu-enkephalin. J. Pept. Sci. 4, 253–265.</ref>. | The main conformations of enkephalin found in crystals have been classified in three categories, described as “extended,” “single bend,” and “double bend.”<ref>Deschamps J. R., George C., Flippen-Anderson J. L. (1996). Structural studies of opioid peptides: a review of recent progress in x-ray diffraction studies. Biopolymers 40, 121–139. 10.1002/bip.360400102</ref>. The picture below represents molecular models of Leu-enkephalin in the three main conformations found in solid state determinations when the peptide is dissolved in a DMSO/water cryomixture at 275 K. <ref>Amodeo P., Naider F., Picone D., Tancredi T., Temussi P. A. (1998). Conformational sampling of bioactive conformers: a low temperature NMR study of 15N-Leu-enkephalin. J. Pept. Sci. 4, 253–265.</ref>. | ||
[[Image:Leu-Enkephalin_conformations.jpg]] | [[Image:Leu-Enkephalin_conformations.jpg / thumb]] | ||
Met-enkephalin amino acid sequence is Tyr-Gly-Gly-Phe-Met, while leu-enkephalin amino acid sequence is Tyr-Gly-Gly-Phe-Leu. As we can see, <scene name='89/897677/Tyr1_and_phe4/1'>tyrosine and phenylalanine rings</scene> of leu-enkephalin are on opposite sides of the backbone and point in different directions. A similar conformation was found for met-enkephalin in Bic/PG <ref name="marcotte"/>. | Met-enkephalin amino acid sequence is Tyr-Gly-Gly-Phe-Met, while leu-enkephalin amino acid sequence is Tyr-Gly-Gly-Phe-Leu. As we can see, <scene name='89/897677/Tyr1_and_phe4/1'>tyrosine and phenylalanine rings</scene> of leu-enkephalin are on opposite sides of the backbone and point in different directions. A similar conformation was found for met-enkephalin in Bic/PG <ref name="marcotte"/>. | ||