1ft6: Difference between revisions

Revision as of 16:44, 2 May 2008

REDUCED STATE OF CYTOCHROME C554 FROM NITROSOMONAS EUROPAEA

OverviewOverview

Cytochrome c554 (cyt c554) is a tetra-heme cytochrome involved in the oxidation of NH3 by Nitrosomonas europaea. The X-ray crystal structures of both the oxidized and dithionite-reduced states of cyt c554 in a new, rhombohedral crystal form have been solved by molecular replacement, at 1.6 A and 1.8 A resolution, respectively. Upon reduction, the conformation of the polypeptide chain changes between residues 175 and 179, which are adjacent to hemes III and IV. Cyt c554 displays conserved heme-packing motifs that are present in other heme-containing proteins. Comparisons to hydroxylamine oxidoreductase, the electron donor to cyt c554, and cytochrome c nitrite reductase, an enzyme involved in nitrite ammonification, reveal substantial structural similarity in the polypeptide chain surrounding the heme core environment. The structural determinants of these heme-packing motifs extend to the buried water molecules that hydrogen bond to the histidine ligands to the heme iron. In the original structure determination of a tetragonal crystal form, a cis peptide bond between His129 and Phe130 was identified that appeared to be stabilized by crystal contacts. In the rhombohedral crystal form used in the present high-resolution structure determination, this peptide bond adopts the trans conformation, but with disallowed angles of phi and psi.

About this StructureAbout this Structure

1FT6 is a Single protein structure of sequence from Nitrosomonas europaea. Full crystallographic information is available from OCA.

ReferenceReference

High-resolution structures of the oxidized and reduced states of cytochrome c554 from Nitrosomonas europaea., Iverson TM, Arciero DM, Hooper AB, Rees DC, J Biol Inorg Chem. 2001 Apr;6(4):390-7. PMID:11372197 Page seeded by OCA on Fri May 2 16:44:20 2008

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OCA

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 [[Image:1ft6.jpg|left|200px]]
- {{Structure
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-}}
 '''REDUCED STATE OF CYTOCHROME C554 FROM NITROSOMONAS EUROPAEA'''
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 [[Category: Iverson, T M.]]
 [[Category: Rees, D C.]]
-[[Category: heme-stacking]]
+[[Category: Heme-stacking]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 16:44:20 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 20:29:52 2008''