1fos: Difference between revisions

Revision as of 16:35, 2 May 2008

TWO HUMAN C-FOS:C-JUN:DNA COMPLEXES

OverviewOverview

The Fos and Jun families of eukaryotic transcription factors heterodimerize to form complexes capable of binding 5'-TGAGTCA-3' DNA elements. We have determined the X-ray crystal structure of a heterodimer of the bZIP regions of c-Fos and c-Jun bound to DNA. Both subunits form continuous alpha-helices. The carboxy-terminal regions form an asymmetric coiled-coil, and the amino-terminal regions make base-specific contacts with DNA in the major groove. Comparison of the two crystallographically distinct protein-DNA complexes show that the coiled-coil is flexibly joined to the basic regions and that the Fos-Jun heterodimer does not recognize the asymmetric 5'-TGAGTCA-3' recognition element in a unique orientation. There is an extensive network of electrostatic interactions between subunits within the coiled-coil, consistent with proposals that these interactions determine preferential formation of the heterodimer over either of the homodimers.

About this StructureAbout this Structure

1FOS is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.

ReferenceReference

Crystal structure of the heterodimeric bZIP transcription factor c-Fos-c-Jun bound to DNA., Glover JN, Harrison SC, Nature. 1995 Jan 19;373(6511):257-61. PMID:7816143 Page seeded by OCA on Fri May 2 16:35:14 2008

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OCA

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 [[Image:1fos.gif|left|200px]]
- {{Structure
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 '''TWO HUMAN C-FOS:C-JUN:DNA COMPLEXES'''
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 [[Category: Glover, J N.M.]]
 [[Category: Harrison, S C.]]
-[[Category: coiled-coil]]
+[[Category: Coiled-coil]]
-[[Category: dna-binding protein]]
+[[Category: Dna-binding protein]]
-[[Category: heterodimer]]
+[[Category: Heterodimer]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 16:35:14 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 20:27:12 2008''