1fn4: Difference between revisions

Revision as of 16:31, 2 May 2008

CRYSTAL STRUCTURE OF FAB198, AN EFFICIENT PROTECTOR OF ACETYLCHOLINE RECEPTOR AGAINST MYASTHENOGENIC ANTIBODIES

OverviewOverview

The crystal structure of the Fab fragment of the rat monoclonal antibody 198, with protective activity for the main immunogenic region of the human muscle acetylcholine receptor against the destructive action of myasthenic antibodies, has been determined and refined to 2.8 A resolution by X-ray crystallographic methods. The mouse anti-lysozyme Fab D1.3 was used as a search model in molecular replacement with the AMORE software. The complementarity determining regions (CDR)-L2, CDR-H1 and CDR-H2 belong to canonical groups. Loops CDR-L3, CDR-H2 and CDR-H3, which seem to make a major contribution to binding, were analyzed and residues of potential importance for antigen-binding are examined. The antigen-binding site was found to be a long crescent-shaped crevice. The structure should serve as a model in the rational design of very high affinity humanized mutants of Fab198, appropriate for therapeutic approaches in the model autoimmune disease myasthenia gravis.

About this StructureAbout this Structure

Full crystallographic information is available from OCA.

ReferenceReference

Crystal structure of Fab198, an efficient protector of the acetylcholine receptor against myasthenogenic antibodies., Poulas K, Eliopoulos E, Vatzaki E, Navaza J, Kontou M, Oikonomakos N, Acharya KR, Tzartos SJ, Eur J Biochem. 2001 Jul;268(13):3685-93. PMID:11432734 Page seeded by OCA on Fri May 2 16:31:31 2008

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OCA

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 [[Image:1fn4.gif|left|200px]]
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 '''CRYSTAL STRUCTURE OF FAB198, AN EFFICIENT PROTECTOR OF ACETYLCHOLINE RECEPTOR AGAINST MYASTHENOGENIC ANTIBODIES'''
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 ==About this Structure==
-FN4 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FN4 OCA].
+Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FN4 OCA].
 ==Reference==
 Crystal structure of Fab198, an efficient protector of the acetylcholine receptor against myasthenogenic antibodies., Poulas K, Eliopoulos E, Vatzaki E, Navaza J, Kontou M, Oikonomakos N, Acharya KR, Tzartos SJ, Eur J Biochem. 2001 Jul;268(13):3685-93. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11432734 11432734]
-[[Category: Protein complex]]
-[[Category: Rattus norvegicus]]
 [[Category: Eliopoulos, E.]]
 [[Category: Kontou, M.]]
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 [[Category: Poulas, K.]]
 [[Category: Vatzaki, E.]]
-[[Category: fab198]]
+[[Category: Fab198]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 16:31:31 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 20:26:19 2008''