Lysine-cysteine NOS bonds: Difference between revisions

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Eric Martz, Michal Harel

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 ==Prevalence==
-A survey of the data in the [[Protein Data Bank]] revealed that the NOS bond likely exists "in diverse protein families across all domains of life (including ''Homo sapiens'') and that it is often located at catalytic or regulatory hotspots."<ref name="wensien2021" /> Because the NOS bond was unknown before 2021, it could easily have been overlooked in earlier interpretations of [[electron density maps]].<ref name="wensien2021" /> Three examples are given illustrating putative NOS bonds in active sites of enzymes which, if correct, were previously overlooked:
+A survey of the data in the [[Protein Data Bank]] revealed that the NOS bond likely exists "in diverse protein families across all domains of life (including ''Homo sapiens'') and that it is often located at catalytic or regulatory hotspots."<ref name="wensien2021" /> They found that roughly 0.3% of entries in the PDB with resolutions of 2.0 Å or betterd have Lys-Cys NOS bonds<ref name="preprint">NOS and SONOS redox switches in proteins, von Pappenheim, Wensien, Ye, Uranga, Funk, Mata & Tittmann, preprint posted July, 2021. [https://doi.org/10.21203/rs.3.rs-635771/v1 DOI 10.21203/rs.3.rs-635771/v1].</ref>. Because the NOS bond was unknown before 2019, it was often overlooked in earlier interpretations of [[electron density maps]].<ref name="wensien2021" /> Three examples are given illustrating putative NOS bonds in active sites of enzymes which, if correct, were previously overlooked:
 *[[1m3q]] 2004, a DNA glycosylase: Lys249 – Cys253.
 *[[5y72]] 2018, a prenyltransferase: Lys275 – Cys223.