Lysine-cysteine NOS bonds: Difference between revisions
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<StructureSection size='350' side='right' caption='' scene='88/883792/6zx4_nos/2'> | <StructureSection size='350' side='right' caption='' scene='88/883792/6zx4_nos/2'> | ||
Covalent Lysine-Cysteine protein crosslinks were first reported in 2016, interpreted at the time as Lys-CH<sub>2</sub>-Cys<ref name="methylene">PMID: 27261771</ref><ref name="matthews">PMID: 34180568</ref>. In 2019, Jimin Wang provided evidence for oxygen rather than methylene as the linker<ref name="jimin">PMID: 30592103</ref><ref name="dauterletter">PMID: 30666728</ref>. In 2021, Wensien ''et al.'' with the Tittmann group (Goettingen), studying transaldolases, put the Lysine-cysteine "Nitrogen-Oxygen-Sulfur" (NOS) protein crosslink on firm ground (<scene name='88/883792/6zx4_nos/2'>restore initial scene</scene>)<ref name="wensien2021">PMID: 33953398</ref><ref name="nandv">PMID: 33953388</ref>. The sidechains of a lysine and a cysteine, joined by an NOS bond, make a covalent linkage between polypeptide chains. For comparison, the [[disulfide bond]] is a far more common type of [[Protein crosslinks|covalent linkage between polypeptide chains]], and the [[isopeptide bond]] is another rare type. | Covalent Lysine-Cysteine protein crosslinks were first reported in 2016, interpreted at the time as Lys-CH<sub>2</sub>-Cys<ref name="methylene">PMID: 27261771</ref><ref name="matthews">PMID: 34180568</ref>. In 2019, Jimin Wang provided evidence for oxygen rather than methylene as the linker<ref name="jimin">PMID: 30592103</ref><ref name="dauterletter">PMID: 30666728</ref>. In 2021, Wensien ''et al.'' with the Tittmann group (Goettingen), studying transaldolases, put the Lysine-cysteine "Nitrogen-Oxygen-Sulfur" (NOS) protein crosslink on firm ground (<scene name='88/883792/6zx4_nos/2'>restore initial scene</scene>)<ref name="wensien2021">PMID: 33953398</ref><ref name="nandv">PMID: 33953388</ref>. The sidechains of a lysine and a cysteine, joined by an NOS bond, make a covalent linkage between polypeptide chains. For comparison, the [[disulfide bond]] is a far more common type of [[Protein crosslinks|covalent linkage between polypeptide chains]], and the [[isopeptide bond]] is another rare type. | ||
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*Oxidized: NOS bond present, enzyme inactive. | *Oxidized: NOS bond present, enzyme inactive. | ||
</StructureSection> | |||
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==Prevalence== | ==Prevalence== | ||
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*[[5y72]], a prenyltransferase: Lys275 – Cys223. | *[[5y72]], a prenyltransferase: Lys275 – Cys223. | ||
*[[6t3x]], a cytomegalovirus nuclear egress protein: Lys132 – Cys54. | *[[6t3x]], a cytomegalovirus nuclear egress protein: Lys132 – Cys54. | ||
==Methods== | ==Methods== | ||