7oo4: Difference between revisions

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 ==HaloTag Engineering for Enhanced Fluorogenicity and Kinetics with a Styrylpyridine Dye==
-<StructureSection load='7oo4' size='340' side='right'caption='[[7oo4]]' scene=''>
+<StructureSection load='7oo4' size='340' side='right'caption='[[7oo4]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7OO4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7OO4 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[7oo4]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Rhoso Rhoso]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7OO4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7OO4 FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7oo4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7oo4 OCA], [https://pdbe.org/7oo4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7oo4 RCSB], [https://www.ebi.ac.uk/pdbsum/7oo4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7oo4 ProSAT]</span></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=VF2:4-[(E)-2-[1-(7-chloranylheptyl)pyridin-1-ium-4-yl]ethenyl]-N,N-dimethyl-aniline'>VF2</scene></td></tr>
+<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">dhaA ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1831 RHOSO])</td></tr>
+<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Haloalkane_dehalogenase Haloalkane dehalogenase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.8.1.5 3.8.1.5] </span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7oo4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7oo4 OCA], [https://pdbe.org/7oo4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7oo4 RCSB], [https://www.ebi.ac.uk/pdbsum/7oo4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7oo4 ProSAT]</span></td></tr>
 </table>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+HaloTag is a small self-labeling protein that is frequently used for creating fluorescent reporters in living cells. The small-molecule dyes used with HaloTag are almost exclusively based on rhodamine scaffolds, which are often expensive and challenging to synthesize. Herein, we report the engineering of HaloTag for use with a chemically accessible, inexpensive fluorophore based on the dimethylamino-styrylpyridium dye. Through directed evolution, the maximum fluorogenicity and the apparent second-order bioconjugation rate constants could be improved up to 4-fold and 42-fold, respectively. One of the top variants, HT-SP5, enabled reliable imaging in mammalian cells, with a 113-fold fluorescence enhancement over the parent protein. Additionally, crystallographic characterization of selected mutants suggests the chemical origin of the fluorescent enhancement. The improved dye system offers a valuable tool for imaging and illustrates the viability of engineering self-labeling proteins for alternative fluorophores.
+HaloTag Engineering for Enhanced Fluorogenicity and Kinetics with a Styrylpyridium Dye.,Miro-Vinyals C, Stein A, Fischer S, Ward TR, Deliz Liang A Chembiochem. 2021 Oct 5. doi: 10.1002/cbic.202100424. PMID:34609782<ref>PMID:34609782</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+<div class="pdbe-citations 7oo4" style="background-color:#fffaf0;"></div>
+== References ==
+<references/>
 __TOC__
 </StructureSection>
+[[Category: Haloalkane dehalogenase]]
 [[Category: Large Structures]]
-[[Category: Liang AD]]
+[[Category: Rhoso]]
-[[Category: Stein A]]
+[[Category: Liang, A D]]
+[[Category: Stein, A]]
+[[Category: Channel dye]]
+[[Category: Directed evolution]]
+[[Category: Fluorescence]]
+[[Category: Fluorescent protein]]
+[[Category: Fluorescent reporter]]
+[[Category: Halotag]]
+[[Category: Imaging]]
+[[Category: Mammalian cell]]