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==X-ray crystal structure of a cyclic peptide containing beta-2-microglobulin (63-69) and a gamma-methylornithine turn unit== | ==X-ray crystal structure of a cyclic peptide containing beta-2-microglobulin (63-69) and a gamma-methylornithine turn unit== | ||
<StructureSection load='7lib' size='340' side='right'caption='[[7lib]]' scene=''> | <StructureSection load='7lib' size='340' side='right'caption='[[7lib]], [[Resolution|resolution]] 1.10Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7LIB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7LIB FirstGlance]. <br> | <table><tr><td colspan='2'>[[7lib]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7LIB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7LIB FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7lib FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7lib OCA], [https://pdbe.org/7lib PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7lib RCSB], [https://www.ebi.ac.uk/pdbsum/7lib PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7lib ProSAT]</span></td></tr> | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MVA:N-METHYLVALINE'>MVA</scene>, <scene name='pdbligand=ORN:L-ORNITHINE'>ORN</scene>, <scene name='pdbligand=PHI:IODO-PHENYLALANINE'>PHI</scene>, <scene name='pdbligand=Y1V:'>Y1V</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7lib FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7lib OCA], [https://pdbe.org/7lib PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7lib RCSB], [https://www.ebi.ac.uk/pdbsum/7lib PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7lib ProSAT]</span></td></tr> | |||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Although beta-hairpins are widespread in proteins, there is still no universal tool to coax any small peptide to adopt a beta-hairpin conformation, regardless of sequence. Here, we report that delta-linked gamma( R )-methyl-ornithine ( delta MeOrn) provides an improved beta-turn template for inducing a beta-hairpin conformation in peptides. We have developed a synthesis of protected delta MeOrn as a building block suitable for use in Fmoc-based solid-phase peptide synthesis. The synthesis begins with l -leucine and affords gram quantities of the N alpha -Boc- N delta -Fmoc-gamma( R )-methyl-ornithine building block. X-ray crystallography confirms that the delta MeOrn turn unit adopts a folded structure in a macrocyclic beta-hairpin peptide. CD and NMR spectroscopic experiments allow comparison of the delta MeOrn turn template to the delta-linked ornithine ( delta Orn) turn template that our laboratory has previously introduced and also to the popular d -Pro-Gly turn template. Collectively, these studies demonstrate that the folding of the delta MeOrn turn template is substantially better than that of delta Orn and is comparable to d -Pro-Gly. | |||
An Improved Turn Structure for Inducing beta-Hairpin Formation in Peptides.,Nowick JS, Li X, Sabol AL, Wierzbicki M, Salveson PJ Angew Chem Int Ed Engl. 2021 Jul 13. doi: 10.1002/anie.202105559. PMID:34258835<ref>PMID:34258835</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 7lib" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Li X]] | [[Category: Li, X]] | ||
[[Category: Nowick | [[Category: Nowick, J S]] | ||
[[Category: Wierzbicki M]] | [[Category: Wierzbicki, M]] | ||
[[Category: Beta-2-microglobulin]] | |||
[[Category: De novo protein]] | |||
[[Category: Turn unit]] | |||
Revision as of 23:14, 20 October 2021
X-ray crystal structure of a cyclic peptide containing beta-2-microglobulin (63-69) and a gamma-methylornithine turn unitX-ray crystal structure of a cyclic peptide containing beta-2-microglobulin (63-69) and a gamma-methylornithine turn unit
Structural highlights
Publication Abstract from PubMedAlthough beta-hairpins are widespread in proteins, there is still no universal tool to coax any small peptide to adopt a beta-hairpin conformation, regardless of sequence. Here, we report that delta-linked gamma( R )-methyl-ornithine ( delta MeOrn) provides an improved beta-turn template for inducing a beta-hairpin conformation in peptides. We have developed a synthesis of protected delta MeOrn as a building block suitable for use in Fmoc-based solid-phase peptide synthesis. The synthesis begins with l -leucine and affords gram quantities of the N alpha -Boc- N delta -Fmoc-gamma( R )-methyl-ornithine building block. X-ray crystallography confirms that the delta MeOrn turn unit adopts a folded structure in a macrocyclic beta-hairpin peptide. CD and NMR spectroscopic experiments allow comparison of the delta MeOrn turn template to the delta-linked ornithine ( delta Orn) turn template that our laboratory has previously introduced and also to the popular d -Pro-Gly turn template. Collectively, these studies demonstrate that the folding of the delta MeOrn turn template is substantially better than that of delta Orn and is comparable to d -Pro-Gly. An Improved Turn Structure for Inducing beta-Hairpin Formation in Peptides.,Nowick JS, Li X, Sabol AL, Wierzbicki M, Salveson PJ Angew Chem Int Ed Engl. 2021 Jul 13. doi: 10.1002/anie.202105559. PMID:34258835[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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