1fhy: Difference between revisions

Revision as of 16:20, 2 May 2008

PSORALEN CROSS-LINKED D(CCGCTAGCGG) FORMS HOLLIDAY JUNCTION

OverviewOverview

The single-crystal structures are presented for two DNA sequences with the thymine bases covalently cross-linked across the complementary strands by 4'-hydroxymethyl-4,5',8-trimethylpsoralen (HMT). The HMT-adduct of d(CCGCTAGCGG) forms a psoralen-induced Holliday junction, showing for the first time the effect of this important class of chemotheraputics on the structure of the recombination intermediate. In contrast, HMT-d(CCGGTACCGG) forms a sequence-dependent junction. In both structures, the DNA duplex is highly distorted at the thymine base linked to the six-member pyrone ring of the drug. The psoralen cross-link defines the intramolecular interactions of the drug-induced junction, while the sequence-dependent structure is nearly identical to the native Holliday junction of d(CCGGTACCGG) alone. The two structures contrast the effects of drug- and sequence-dependent interactions on the structure of a Holliday junction, suggesting a role for psoralen in the mechanism to initiate repair of psoralen-lesions in mammalian DNA.

About this StructureAbout this Structure

Full crystallographic information is available from OCA.

ReferenceReference

The crystal structures of psoralen cross-linked DNAs: drug-dependent formation of Holliday junctions., Eichman BF, Mooers BH, Alberti M, Hearst JE, Ho PS, J Mol Biol. 2001 Apr 20;308(1):15-26. PMID:11302703 Page seeded by OCA on Fri May 2 16:20:48 2008

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OCA

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 [[Image:1fhy.gif|left|200px]]
- {{Structure
+<!--
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+The line below this paragraph, containing "STRUCTURE_1fhy", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=DA:2&#39;-DEOXYADENOSINE-5&#39;-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2&#39;-DEOXYCYTIDINE-5&#39;-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2&#39;-DEOXYGUANOSINE-5&#39;-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5&#39;-MONOPHOSPHATE'>DT</scene>, <scene name='pdbligand=PSO:4&#39;-HYDROXYMETHYL-4,5&#39;,8-TRIMETHYLPSORALEN'>PSO</scene>
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+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
-|DOMAIN=
+{{STRUCTURE_1fhy|  PDB=1fhy  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1fhy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fhy OCA], [http://www.ebi.ac.uk/pdbsum/1fhy PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1fhy RCSB]</span>
-}}
 '''PSORALEN CROSS-LINKED D(CCGCTAGCGG) FORMS HOLLIDAY JUNCTION'''
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 ==About this Structure==
-FHY is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FHY OCA].
+Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FHY OCA].
 ==Reference==
 The crystal structures of psoralen cross-linked DNAs: drug-dependent formation of Holliday junctions., Eichman BF, Mooers BH, Alberti M, Hearst JE, Ho PS, J Mol Biol. 2001 Apr 20;308(1):15-26. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11302703 11302703]
-[[Category: Protein complex]]
 [[Category: Alberti, M.]]
 [[Category: Eichman, B F.]]
@@ Line 30: / Line 26: @@
 [[Category: Mooers, B H.M.]]
 [[Category: 4-way junction]]
-[[Category: cross-linked dna]]
+[[Category: Cross-linked dna]]
-[[Category: dna-drug complex]]
+[[Category: Dna-drug complex]]
-[[Category: holliday junction]]
+[[Category: Holliday junction]]
-[[Category: psoralen]]
+[[Category: Psoralen]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 16:20:48 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 20:23:15 2008''