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| ==X-RAY STRUCTURE OF THE RNA-BINDING PROTEIN SHE2p== | | ==X-RAY STRUCTURE OF THE RNA-BINDING PROTEIN SHE2p== |
| <StructureSection load='1xly' size='340' side='right'caption='[[1xly]], [[Resolution|resolution]] 1.95Å' scene=''> | | <StructureSection load='1xly' size='340' side='right'caption='[[1xly]]' scene=''> |
| == Structural highlights == | | == Structural highlights == |
| <table><tr><td colspan='2'>[[1xly]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XLY OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1XLY FirstGlance]. <br> | | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XLY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1XLY FirstGlance]. <br> |
| </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SHE2, YKL130C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824])</td></tr> | | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1xly FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xly OCA], [https://pdbe.org/1xly PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1xly RCSB], [https://www.ebi.ac.uk/pdbsum/1xly PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1xly ProSAT]</span></td></tr> |
| <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1xly FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xly OCA], [http://pdbe.org/1xly PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1xly RCSB], [http://www.ebi.ac.uk/pdbsum/1xly PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1xly ProSAT]</span></td></tr> | |
| </table> | | </table> |
| == Function ==
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| [[http://www.uniprot.org/uniprot/SHE2_YEAST SHE2_YEAST]] RNA-binding protein that binds specific mRNAs including the ASH1 mRNA, coding for a repressor of the HO endonuclease. Part of the mRNA localization machinery that restricts accumulation of certain proteins to the bud and in the daughter cell. Recruits the MYO4-SHE3 complex to the ASH1 mRNA. Recruites also LOC1 and PUF6 to ASH1 mRNA, which are required for translational repression of this mRNA.<ref>PMID:10212145</ref> <ref>PMID:10359695</ref> <ref>PMID:11032818</ref> <ref>PMID:11101531</ref> <ref>PMID:12499354</ref> <ref>PMID:13679573</ref> <ref>PMID:14561888</ref> <ref>PMID:14691136</ref> <ref>PMID:15328357</ref> <ref>PMID:15537539</ref> <ref>PMID:15899876</ref> <ref>PMID:16890529</ref> <ref>PMID:18566598</ref> <ref>PMID:19244342</ref> <ref>PMID:20713510</ref> <ref>PMID:9809065</ref>
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| == Evolutionary Conservation == | | == Evolutionary Conservation == |
| [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1xly ConSurf]. | | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1xly ConSurf]. |
| <div style="clear:both"></div> | | <div style="clear:both"></div> |
| <div style="background-color:#fffaf0;">
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| == Publication Abstract from PubMed ==
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| Selective transport of mRNAs in ribonucleoprotein particles (mRNP) ensures asymmetric distribution of information within and among eukaryotic cells. Actin-dependent transport of ASH1 mRNA in yeast represents one of the best-characterized examples of mRNP translocation. Formation of the ASH1 mRNP requires recognition of zip code elements by the RNA binding protein She2p. We determined the X-ray structure of She2p at 1.95 A resolution. She2p is a member of a previously unknown class of nucleic acid binding proteins, composed of a single globular domain with a five alpha helix bundle that forms a symmetric homodimer. After demonstrating potent, dimer-dependent RNA binding in vitro, we mapped the RNA binding surface of She2p to a basic helical hairpin in vitro and in vivo and present a mechanism for mRNA-dependent initiation of ASH1 mRNP complex assembly.
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| She2p is a novel RNA binding protein with a basic helical hairpin motif.,Niessing D, Huttelmaier S, Zenklusen D, Singer RH, Burley SK Cell. 2004 Nov 12;119(4):491-502. PMID:15537539<ref>PMID:15537539</ref>
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| From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| </div>
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| <div class="pdbe-citations 1xly" style="background-color:#fffaf0;"></div>
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| == References ==
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| <references/>
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| __TOC__ | | __TOC__ |
| </StructureSection> | | </StructureSection> |
| [[Category: Atcc 18824]]
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| [[Category: Large Structures]] | | [[Category: Large Structures]] |
| [[Category: Burley, S K]] | | [[Category: Burley SK]] |
| [[Category: Huettelmaier, S]] | | [[Category: Huettelmaier S]] |
| [[Category: Niessing, D]] | | [[Category: Niessing D]] |
| [[Category: Singer, R H]] | | [[Category: Singer RH]] |
| [[Category: Zenklusen, D]] | | [[Category: Zenklusen D]] |
| [[Category: Basic helical hairpin]]
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| [[Category: Dimer]]
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| [[Category: Five helix bundle]]
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| [[Category: Rna binding protein]]
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| [[Category: Rna-binding protein]]
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