1fe0: Difference between revisions

Revision as of 16:13, 2 May 2008

CRYSTAL STRUCTURE OF CADMIUM-HAH1

OverviewOverview

The Hah1 metallochaperone protein is implicated in copper delivery to the Menkes and Wilson disease proteins. Hah1 and the N-termini of its target proteins belong to a family of metal binding domains characterized by a conserved MT/HCXXC sequence motif. The crystal structure of Hah1 has been determined in the presence of Cu(I), Hg(II), and Cd(II). The 1.8 A resolution structure of CuHah1 reveals a copper ion coordinated by Cys residues from two adjacent Hah1 molecules. The CuHah1 crystal structure is the first of a copper chaperone bound to copper and provides structural support for direct metal ion exchange between conserved MT/HCXXC motifs in two domains. The structures of HgHah1 and CdHah1, determined to 1.75 A resolution, also reveal metal ion coordination by two MT/HCXXC motifs. An extended hydrogen bonding network, unique to the complex of two Hah1 molecules, stabilizes the metal binding sites and suggests specific roles for several conserved residues. Taken together, the structures provide models for intermediates in metal ion transfer and suggest a detailed molecular mechanism for protein recognition and metal ion exchange between MT/HCXXC containing domains.

About this StructureAbout this Structure

1FE0 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

ReferenceReference

Structural basis for copper transfer by the metallochaperone for the Menkes/Wilson disease proteins., Wernimont AK, Huffman DL, Lamb AL, O'Halloran TV, Rosenzweig AC, Nat Struct Biol. 2000 Sep;7(9):766-71. PMID:10966647 Page seeded by OCA on Fri May 2 16:13:07 2008

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OCA

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- {{Structure
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-}}
 '''CRYSTAL STRUCTURE OF CADMIUM-HAH1'''
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 [[Category: Rosenzweig, A C.]]
 [[Category: Wernimont, A K.]]
-[[Category: beta-alpha-beta-beta-alpha-beta]]
+[[Category: Beta-alpha-beta-beta-alpha-beta]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 16:13:07 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 20:21:08 2008''