1fdn: Difference between revisions

Revision as of 16:12, 2 May 2008

REFINED CRYSTAL STRUCTURE OF THE 2[4FE-4S] FERREDOXIN FROM CLOSTRIDIUM ACIDURICI AT 1.84 ANGSTROMS RESOLUTION

OverviewOverview

The crystal structure of the 2[4Fe-4S] ferredoxin from Clostridium acidurici has been determined at a resolution of 1.84 A and refined to an R-factor of 0.169. Crystals belong to space group P4(3)2(1)2 with unit cell dimensions a = b = 34.44 A and c = 74.78 A. The structure was determined by molecular replacement using the previously published model of an homologous ferredoxin and refined by molecular dynamics techniques. The model contains the protein and 46 water molecules. Only two amino acid residues, Asp27 and Asp28, are poorly defined in the electron density maps. The molecule has an overall chain fold similar to that of other [4Fe-4S] bacterial ferredoxins of known structure. The two [4Fe-4S] clusters display similar bond distances and angles. In both of them the co-ordination of one iron atom (bound to Cys11 and Cys40) is slightly distorted as compared with that of the other iron atoms. A core of hydrophobic residues and a few water molecules contribute to the stability of the structure. The [4Fe-4S] clusters interact with the polypeptide chain through eight hydrogen bonds each, in addition to the covalent Fe-Scys bonds. The ferredoxin from Clostridium acidurici is the most typical clostridial ferredoxin crystallized so far and the biological implications of the newly determined structure are discussed.

About this StructureAbout this Structure

1FDN is a Single protein structure of sequence from Clostridium acidurici. Full crystallographic information is available from OCA.

ReferenceReference

Refined crystal structure of the 2[4Fe-4S] ferredoxin from Clostridium acidurici at 1.84 A resolution., Duee ED, Fanchon E, Vicat J, Sieker LC, Meyer J, Moulis JM, J Mol Biol. 1994 Nov 4;243(4):683-95. PMID:7966291 Page seeded by OCA on Fri May 2 16:12:24 2008

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OCA

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 '''REFINED CRYSTAL STRUCTURE OF THE 2[4FE-4S] FERREDOXIN FROM CLOSTRIDIUM ACIDURICI AT 1.84 ANGSTROMS RESOLUTION'''
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 [[Category: Sieker, L C.]]
 [[Category: Vicat, J.]]
-[[Category: electron transport]]
+[[Category: Electron transport]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 16:12:24 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 20:20:51 2008''