1fdi: Difference between revisions

Revision as of 16:12, 2 May 2008

OXIDIZED FORM OF FORMATE DEHYDROGENASE H FROM E. COLI COMPLEXED WITH THE INHIBITOR NITRITE

OverviewOverview

Formate dehydrogenase H from Escherichia coli contains selenocysteine (SeCys), molybdenum, two molybdopterin guanine dinucleotide (MGD) cofactors, and an Fe4S4 cluster at the active site and catalyzes the two-electron oxidation of formate to carbon dioxide. The crystal structures of the oxidized [Mo(VI), Fe4S4(ox)] form of formate dehydrogenase H (with and without bound inhibitor) and the reduced [Mo(IV), Fe4S4(red)] form have been determined, revealing a four-domain alphabeta structure with the molybdenum directly coordinated to selenium and both MGD cofactors. These structures suggest a reaction mechanism that directly involves SeCys140 and His141 in proton abstraction and the molybdenum, molybdopterin, Lys44, and the Fe4S4 cluster in electron transfer.

About this StructureAbout this Structure

1FDI is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

ReferenceReference

Crystal structure of formate dehydrogenase H: catalysis involving Mo, molybdopterin, selenocysteine, and an Fe4S4 cluster., Boyington JC, Gladyshev VN, Khangulov SV, Stadtman TC, Sun PD, Science. 1997 Feb 28;275(5304):1305-8. PMID:9036855 Page seeded by OCA on Fri May 2 16:12:06 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
 [[Image:1fdi.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 1fdi |SIZE=350|CAPTION= <scene name='initialview01'>1fdi</scene>, resolution 2.9&Aring;
+The line below this paragraph, containing "STRUCTURE_1fdi", creates the "Structure Box" on the page.
-|SITE= <scene name='pdbsite=FS4:The+Fe+S+Cluster+Is+Coordinated+To+The+S+Atoms+Of+CYS+8,+...'>FS4</scene> and <scene name='pdbsite=MO4:The+Mo+Atom+Is+Coordinated+To+The+Se+Atom+Of+Sec+140,+Th+...'>MO4</scene>
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=6MO:MOLYBDENUM(VI)+ION'>6MO</scene>, <scene name='pdbligand=CSE:SELENOCYSTEINE'>CSE</scene>, <scene name='pdbligand=MGD:2-AMINO-5,6-DIMERCAPTO-7-METHYL-3,7,8A,9-TETRAHYDRO-8-OXA-1,3,9,10-TETRAAZA-ANTHRACEN-4-ONE+GUANOSINE+DINUCLEOTIDE'>MGD</scene>, <scene name='pdbligand=NO2:NITRITE+ION'>NO2</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Formate_dehydrogenase Formate dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.2.1.2 1.2.1.2] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE= FDHF ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])
+-->
-|DOMAIN=
+{{STRUCTURE_1fdi|  PDB=1fdi  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1fdi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fdi OCA], [http://www.ebi.ac.uk/pdbsum/1fdi PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1fdi RCSB]</span>
-}}
 '''OXIDIZED FORM OF FORMATE DEHYDROGENASE H FROM E. COLI COMPLEXED WITH THE INHIBITOR NITRITE'''
@@ Line 28: / Line 25: @@
 [[Category: Boyington, J C.]]
 [[Category: Sun, P D.]]
-[[Category: anaerobic]]
+[[Category: Anaerobic]]
-[[Category: dehydrogenase]]
+[[Category: Dehydrogenase]]
-[[Category: fe4s4]]
+[[Category: Fe4s4]]
-[[Category: formate]]
+[[Category: Formate]]
-[[Category: iron sulfur cluster]]
+[[Category: Iron sulfur cluster]]
-[[Category: mgd]]
+[[Category: Mgd]]
-[[Category: molybdenum]]
+[[Category: Molybdenum]]
-[[Category: molybdopterin]]
+[[Category: Molybdopterin]]
-[[Category: molybdopterin guanine dinucleotide]]
+[[Category: Molybdopterin guanine dinucleotide]]
-[[Category: mpt]]
+[[Category: Mpt]]
-[[Category: oxidoreductase]]
+[[Category: Oxidoreductase]]
-[[Category: secy]]
+[[Category: Secy]]
-[[Category: selenium]]
+[[Category: Selenium]]
-[[Category: selenocysteine]]
+[[Category: Selenocysteine]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 16:12:06 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 20:20:50 2008''